Structural Multiplex FFPE Reference Standard

Format
FFPE

Description

The Structural Multiplex FFPE Reference Standard is a highly-characterized, biologically-relevant quality control material, which can be used to assess the performance of NGS assays that detect structural, low-allelic somatic mutations.

With this product, you can:

  • Evaluate workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics
  • Optimize and validate the sensitivity of your workflow for structural variant detection
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay

HD789
$203.00

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Technical Information

The Structural Multiplex Reference Standard covers a wide range of mutations in defined genomic context. This standard is designed to challenge your molecular and bioinformatics workflow by providing validated copy number variants/ amplifications, translocations and large insertions/deletions. Additionally, you may examine the genomic context of variants within regions of specific GC-content (high vs. low). The Structural Multiplex FFPE DNA Reference Standard includes 9 digital PCR-validated variants with allelic frequencies ranging from 3.5% to 9.7% and CNVs at 4.5x and 8.5x amplification.

Highlight features of this control material include RET and ROS1 fusion variants, MYC-N and MET focal amplifications and a BRCA2 variant.

The Structural Multiplex Reference Standard is also available in high molecular weight gDNA (HD753) and cfDNA (HD786) format.

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Technical Data

Genes Covered
AKT1, BRAF, BRCA2, EGFR, FBXW7, FLT3, GNA11, KRAS, MET, MYC-N, NOTCH1, PIK3CA, RET, ROS1
Fixation Method
4% Formalin
Section Size
15 µm
Cell Density
3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield
≥ 400 ng DNA per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit

Product Information

Verified Mutations
Variant Type Chromosome Number Gene Variant Expected Allelic Frequency, %
SNV High GC  chr.19 GNA11 Q209L 4.4
SNV High GC  chr.14 AKT1 E17K 3.5
SNV Low GC  chr.3 PIK3CA E545K 4.5
Long Insertion  chr.7 EGFR V769_D770insASV 4.4
Long Deletion chr.7 EGFR ΔE746 - A750 4.4
Fusion  chr.4:chr.6 ROS1 SLC34A2/ROS1 fusion 9.7
Fusion  chr.10 RET CCDC6/RET fusion 4.6
CNV  chr.7 MET amplification 4.5 copies
CNV  chr.2 MYC-N amplification 8.5 copies
Presence confirmed in parental cell line
Variant TypeChromosome NumberGeneVariant
SNV Low GC chr.12KRASG13D
SNV High GCchr.9NOTCH1P668S
Short Deletionchr.7 METV237fs
Short Deletionchr.13FLT3S985fs
Short Deletionchr.13 BRCA2A1689fs
Short Deletion chr.4FBXW7G667fs
SNVchr.7EGFRG719S
SNVchr.7BRAFV600E
SNVchr.3PIK3CAH1047R
Unit Size
1 FFPE Curl
Extractable DNA
≥ 400 ng DNA per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit
Intended Use
For routine performance monitoring (Research Use Only)

General Information

Allelic Frequency
3.5%-9.7% and 4.5x and 8.5x amplification for the CNVs
Storage
4˚C
Expiry
48 months from the date of manufacture

Quality Control

Allelic Frequency
Droplet Digital™ PCR
Genotype
Droplet Digital™ PCR
Quality
Agarose gel electrophoresis
Quantification
Quantifluor™
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: FFPE-DNA
PDF Download
Batch ID: 31292
PDF Download
Batch ID: 36228
PDF Download
Batch ID: 36229
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Documents & Related Literature

Product Insert
PDF Download
Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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