This model expresses cre-recombinase under the control of the endogenous dopamine transporter promoter enabling specific expression in dopaminergic neurons. This model possesses a targeted insertion of (IRES)-cre immediately after the translational stop in the open reading frame of DAT. The DAT-Cre rat is useful for applications requiring tissue specific expression, including optogenetics and breeding with transgenic floxed lines.
• Optogenetics • DREDD • Expression/knockout of floxed genes
• Cre recombinase driven by endogenous DAT promoter • No observed ectopic expression of cre • Targeted insertion eliminates possible gene disruption that may occur in random insertion technologies such as BAC • Background Strain: Sprague-Dawley