Cell Lines For Studying Drug Targets

Target identification and validation are among the most important steps in developing a new drug. Target validation involves proving that either DNA, RNA, or a protein molecule is directly involved in a disease process and can be a suitable target for development of a new therapeutic drug. To qualify as ‘druggable’, a target must be accessible to the proposed drug molecule, and a measurable biological reaction must be provoked as a consequence of the drug interacting with the target. This reaction may be measured both in vitro and in vivo.

Once the target has been identified, for example by genomic or proteomic investigations, the target is then validated in functional studies. The main strategies involved in target validation are gene knockout studies and direct inhibition of the target by small molecules, peptides, antibodies or any other class of inhibitors. The knockout approach genetically inhibits the target, thereby mimicking the action of the drug and allows a drug to be modelled before it is developed.

Information about established drugs and drug-target interactions is available online via public databases such as DrugBank and the Therapeutic Targets Database. In addition, information on potentially novel drug targets can be found at CenterWatch's, Drugs in Clinical Trials Database, which identifies trends in drug development based on current clinical trials.

The X-MAN® range offers a number of drug target Knockout cell lines ready to ship, see below. Each modified cell line is provided with a non-edited parental control to provide confidence in your results.

In addition, Horizon’s cutting edge Pathfinderscreening platforms and SAGE® in vivo models can be used in target identification and validation projects.

Examples of popular knockout cell lines
ABATABCA1ABCC1ACEACHE
ACVR2AADAADIPOR1ADIPOR2ADK
ADORA2AADORA2BADRA1AALADANXA1
ARASIC1ATP2C1BCHEBLVRB
CA2CACNA1HCACNA2D1CACNA2D2CACNB1
CACNB2CACNB3CACNB4CCKBRCCND1
CHD1CHRNB2CKBCLCN2CNR1
COMTCPT1ACPT2CYP51A1DPP4
EPORESRRGFADS1FASNFGF2
FN1FTLGAAGABBR1GABRA5
GABRB3GANABGANCGGCXGLRB
GSRGSSHMMRHPRT1HTR6
IFNAR1IFNAR2IFNGR1IFNGR2IL11RA
IMPDH1ITGA2BJUNKCNC1KCND2
KCNH2KCNJ11KCNK2KCNQ1KCNQ2
KEAP1KMT2CM6PRMAOAMAP1A
MAP2MAP4MMEMMP11MMP15
MMP16MMP17MMP24MMP25MS4A2
MTRMUTNOS2NR3C2PDE10A
PDE4APDE5APDE7APDE8APDXK
PGFPLAUPLAURPNPPOLE4
PRKAG1PRKAG2PRLRPROS1PSMB10
PSMB8PSMB9PTGER3QPRTRAMP1
RAMP2RARGRFKRXRARXRB
S1PR5SCN1BSCN5ASCNN1DSHMT1
SIGMAR1SLC18A2SLC5A2SMSSOAT1
SQLESRD5A1SYT2THRBTOP1MT
TOP2BTPMTTSPOTUBA1ATUBA4A
TUBB1TUBB4BTUBG1UGCGVAMP1
VEGFAVEGFBVKORC1  

Our cell line range is constantly growing. For our most current list of ready-made cell lines and details of custom made engineering projects you can browse our entire offering via the link below.

Browse Our Cell Line Options


For offline access, many of our popular cell lines that are usually available for immediate delivery are listed in our downloadable our catalog - your gene edited cell line may already have been created!

Horizon Cell Lines Catalog
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