Horizon and Promega Corporation have partnered in a long-term relationship to develop a suite of best-in-class endogenous pathway tag and reporter cell lines for application in functional genomics research and drug discovery and development.
X-MAN™ Reporter Kits enable functional cell-based assays to be carried out at the endogenous level, looking at natural levels of protein and promoter activity. This affords researchers a higher level of confidence that their experimental results are meaningful, more accurately reflecting the changes seen in real disease biology.
Two classes of X-MAN™ Reporter Kits are available:
NanoLuc™ luciferase is a novel, small, bright luciferase reporter. It is one third the size and 100 times brighter than the most commonly used luciferase, firefly. Reporter cell lines involve knock-in of the Promega NanoLuc™ reporter into an endogenous locus for in vitro and in vivo pathway read-outs and protein stability experiments. This brightness allows the interrogation of pathway activity from endogenous genes/ promoters, expanding the range of questions that can be asked and the range of genes that can be studied.
Kits are available in 10, 100, 500, 1,000 ml and custom reporter volume sizes.
NanoLuc™ luciferase detection at endogenous expression levels using HCT116 HIF1A NanoLuc® - PEST promoter fusion cell lines, (A) correlation of cell number with luciferase signal; (B) correlation of concentration of Actinomycin D, a non-specific transcriptional inhibitor, with luciferase signal.
Flexible and efficient system…Wider experimental range…Reliable results
HaloTag® is an extremely flexible reporter, enabling protein imaging and localization (which can be performed in live cells), protein-protein interaction experiments through protein pull-down, or protein purification, all using the same cell line by choosing different ligands with which to interrogate the cell line. This approach avoids the use of antibodies, which are often not available or of poor quality.
Kits are available in 15 and 30 µl reagent volume sizes for imaging ligands - two are available, TMR (for red fluorescing) and Oregon Green), or in 24 reactions for pull-down ligands.
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