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Guide RNA Design and Validation

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Guide RNA Design

One of the most critical requirements for successful CRISPR gene editing is to have high efficiency functional guide RNAs.

Horizon has partnered with Desktop Genetics™ to develop a proprietary in silico CRISPR design platform that combines the best public platforms with Horizon’s expert CRISPR knowledge. The system is used internally by Horizon to identify most suitable guide RNAs for each specific project and a version is available for our customers to use.

gUIDEbook™ CRISPR Design Platform: Coming Soon!

Horizon chooses the best predicted guides and provides you with a report, with the oligos ready to clone yourself or with the RNA guide plasmid ready for transfection. Several cloning plasmids also express Cas9, reducing the number of constructs needed to transfect for successful gene editing.

Guide RNA Validation

It is now generally recognized that in order to have confidence that you are working with a suitably active guide RNA (gRNA),it’s best to design at least 5 gRNAs (10 for dual guides) and test them in cells to make sure you obtain at least one with sufficiently high activity.

We therefore offer a validation service where we test guides in a human or mouse cell line through use of an assay that detects cuts to the genome. The best performing guide(s) are provided cloned into a backbone that expresses Cas9, together with a complete report on the guide design, performance and position relative to the target sequence. Additional plasmid clones beyond the first can also be provided.

Get advice from our gene editing experts!

2 hours free expert scientific support with every guide cloning and validation order

Order GENASSIST™ Products and Services online:

Horizon Discovery is interested in licensing many of the cell lines that are being developed by our customers in return for a royalty. There is no commitment from you to do so and please inform Horizon if you are interested in participating.

Order Guide RNA Design and Validation

Alternatively for more information contact us at info@horizondiscovery.com

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