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The following webcasts are scheduled. Participation is free, so please register to participate.

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Please click here to view our archived webcasts.

Haploid genetic screens to uncover the mechanism of action of drugs

Date: September 8th, 2015 16: 00-16: 45 GMT (London time)

Fifteen years after the completion of Human Genome Project, the function of many human genes remains elusive. One major obstacle for the study of human genes is the diploid nature of the genome: Inactivation of one allele is often insufficient to elicit a phenotype because the second (wild-type) allele can maintain gene function. Recently, near-haploid somatic cells were isolated from human patients and taken into culture. Insertional mutagenesis in these cells enables unbiased “yeast-like” genetic screening in human cells. Screens conducted so far have revealed host factors of viruses, elucidated the mechanism of action of bacterial toxins and uncovered the genes defective in rare inherited diseases. At Horizon Discovery, we employ haploid genetic screening to uncover the mechanism of action of drugs. The webinar will highlight the prerequisites of such genetic screens, show some examples of how they perform and discuss possible pitfalls.

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CRISPR, Genome Editing & the Changing Landscape of Molecular Cell Biology

Date: September 10th, 2015 16: 00-16: 45 GMT (London time)

September 10th - Introduction to CRISPR genome editing. Looking at the origins of CRISPR-Cas9 in bacterial immunity, how it works, and how it has been adapted to genome editing. September 17th - How to plan a CRISPR genome editing experiment to maximize your chances of success Discussing the key considerations when planning a CRISPR genome editing experiment. September 24th - CRISPR modified cell lines – what’s possible and how they will impact your research Exploring through case studies the genomic modifications available to cell biologists, including knockouts, knockins, translocations and endogenous tagging. Speaker - Chris Thorne, PhD | Commercial Marketing Manager Chris has been working at Horizon for four years, during which he has been responsible for the genetic validation of all cell lines in Horizon’s catalogue, has been part of the launch of Horizon’s diagnostic reference materials and has supported hundreds of academic labs as they implement CRISPR genome editing with Horizon’s tools. Prior to Horizon Chris completed his PhD at the University of Liverpool.

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Molecular QC: Using Reference Standards in NGS Pipelines

Date: September 24th, 2015 16: 00-16: 40 GMT (London time)

Key Topics The challenges facing molecular QC in NGS (input, coverage, bioinformatics and reproducibility) The benefits of reference material on the full workflow (DNA extraction through to reporting) How to validate all of your somatic variant parameters for NGS pipelines: the Sensitivity, Specificity, Limit of Detection, Repeatability, Reproducibility and Robustness

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Webinar Series: Using Next Generation Sequencing in Oncology

Date: September 24th, 2015 16: 00-17: 00 GMT (London time)

Webinar Series Webinar 1 – Towards Personalized Medicine – The Role of NGS We’ll look at the role of next generation sequencing (NGS) in personalized medicine, also known as ‘precision medicine’ and how it will be changing over the next 5 years. Find out what other NGS laboratories are doing and how your laboratory can capture value from personalized medicine. Webinar 2 – How to Set-up and Optimize your NGS Assay We’ll discuss the key challenges in setting up and optimizing your next generation sequencing (NGS) panels and platforms. We’ll provide an update on the key regulatory issues surrounding the FDA, including laboratory developed tests (LDTs), and look at the various NGS checklists. Webinar 3 – Best Practices in Handling FFPE Samples in NGS We’ll look at the various best practices in sample handling in next generation sequencing (NGS) and how FFPE samples can be used to optimize and improve the performance of your NGS. We’ll compare the various different DNA extraction techniques and discuss how FFPE samples can be used to improve these processes. Webinar 4 – Validating your NGS Workflow – How do I use Reference Standards? We’ll discuss the various reference materials available on the market and how they can be utilized to validate your NGS workflow.

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