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Horizon provides in-vitro and in-vivo assay development and drug screening services powered by our precision gene-editing, human isogenic cell-line platforms and disease microenvironment assays. Our range of optimized cellular assays in areas such as cell growth, signal transduction, apoptosis, angiogenesis and metastasis are a critical part of Horizon's Discovery Toolbox, and provide value throughout the drug discovery process including:
Horizon provides assay development and drug screening services powered by our precision gene-editing and human isogenic cell-line platforms.
Clients are able to access our patient-relevant X-MAN™ genetically defined cell lines to study how specific cancer genes and mutations affect sensitivity to therapeutic compounds. We also offer research services using standard non-isogenic cancer cell lines to supplement our isogenic cell line offering, and have a bank of over 120 lines ready to use.
Our range of optimized cellular assays has applications at many points during the drug discovery process including:
|Viability (multiple assay readouts)||Apoptosis assays
||3D assays (spheroids, methylcellulose)||siRNA/shRNA knockdown|
|Colony formation (2D)||Immunofluorescence
||RNA / cell pellet / lysate preparation|
|Soft agar (3D)
||DNA damage assays
||Expression profiling (qRTPCR, protein)|
|Combination drug assays||Cellular ELISA
||Tubulin polymerisation assay|
|Cell panel profiling (standard and X-MAN™ isogenic cell lines)||FACS
|Invasion and migration assays||Autophagy assay
||Reactive oxygen species assays|
Of particular value are several assays designed to recreate the tumor microenvironment, enabling analysis of parameters such as hypoxia, 3-dimensional growth, senescence, autophagy and drug resistant pathway responses. These assays, particularly when deploying isogenic X-MAN™ cell lines, clearly demonstrate the role that endogenous mutations play in driving disease and reinforce the importance of placing these cells under tumor relevant conditions in order to illicit the true disease phenotypes and drug responses seen in the clinic.
In combination with CrownBio Incorporated we can provide an integrated solution that can include both in vitro and in vivo studies.
Horizon is also able to develop customized assays specificly designed to address key scientific questions. For example, assays utilizing activity-dead cell lines to determine whether known effect is based on structural or functional causes.
Horizon has successfully completed over 300 projects using isogenic and standard cancer cell line models. Below are just a few examples:
Projects can be carried out on either a fee-for-service (FFS) or full-time equivalent (FTE) basis depending on your requirements.
Below are a few examples and guide prices for FFS projects. Project costs can vary significantly depending on individual project requirements however, so please contact us to discuss your specific needs further.
|Assay Type||Project Specifics||Guide Costs|
|X-MAN™ proliferation assay||2 lines, 5 compounds||$15,000|
|15 lines, 5 compounds||$80,000|
|70 lines, 5 compounds||$185,000|
|Specialist tumor microenvironment||Hypoxia assays, 0.1%, 1% O2||$10,000|
|3D cell panels, 2D vs 3D||$20,000|
Our service provides more than just data points. Our skilled team is drawn from both academic (disease biology) and industrial (drug discovery and development) backgrounds, ensuring that we apply strong scientific rationale and rigor to all projects within the framework of a timeline-driven industrial setting. We have completed over 300 studies ranging from large multi-stage projects for large pharmaceutical companies to small projects for academic researchers.
As the most experienced users of X-MAN™ isogenic cell lines, we can advise our clients on how best to apply them to address a diverse range of research questions. An example of our expertise lies in Horizon’s own successful program which identified and validated HD001, a first-in-class novel kinase that interacts with the K-Ras pathway.
Horizon is in regular contact with customers throughout projects, offering advice, providing milestone updates and presenting a detailed final report at the conclusion. Below are two examples of final reports:
If you would like us to support you through our assay development and drug profiling service, please complete and submit a project questionnaire and we will be in touch within two business days.