Tru-Q 0 is 100% wild type for 40 mutations found in Tru-Q 1, 2, 3, 4, and 7. Four mutations are present at the endogenous level and are listed in the table below. Three of these four endogenous variants (EGFR G719S, BRAF V600E and KRAS G13D) are checked by ddPCR in every batch of Tru-Q 0 to verify the correct blending ratio of wildtype parental cell lines. The 40 engineered variants featured in the Tru-Q product range can be assumed wild type in Tru-Q 0, as this product is blended from non-engineered wild type cell lines. (However, please note that ddPCR confirmation is not carried out on every batch of Tru-Q-0 for the 40 wild type variants.)
|Chromosome Number||Gene||Variant||Expected Allelic Frequency, %|
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