This model expresses cre-recombinase under the control of the endogenous tyrosine hydroxylase promoter enabling specific expression in dopaminergic neurons. This model possesses a targeted insertion of (IRES)-cre immediately after the translational stop in the open reading frame of Th. The TH-Cre rat is useful for applications requiring tissue specific expression, including optogenetics and breeding with transgenic floxed lines.
• Optogenetics • DREADD • Expression/knockout of floxed genes
• No observed ectopic expression of cre • Targeted insertion eliminates possible gene disruption that may occur in random insertion technologies such as BAC • Background Strain: Sprague-Dawley
Figure 1: Cre-recombinase is expressed in TH neurons of TH-Cre rats. TH expression (red) by immunohistochemistry in neurons of the ventral tegmental area (VTA) and substantia nigra (SNCD) is shown in the left panels. In situ hybridization using sense and antisense probes to Cre was also performed (green, center panels). The third panel shows colocalization of TH and Cre in TH-Cre rats but not wild type (WT). DAPI stain in blue.
Figure 2. Colocalization of TH and Cre in olfactory bulb.Cre recombinase driven by endogenous TH promoter