When Horizon pre-defined RNAi Screening Libraries don't match your experimental requirements, you can make your own custom library with ease, speed and economy.
You are able to cherry-pick from our premier siRNA, microRNA, Edit-R CRISPR (KO) crRNA and synthetic sgRNA, CRISPRmod CRISPRa crRNA and synthetic sgRNA and CRISPRmod CRISPRi synthetic sgRNA reagents for human and mouse reagents to create the ideal collection for your particular needs.
Here's how:
- Load gene identifiers for your desired products
- NCBI Gene ID or Gene Symbol
- miRBase mature name or Accession
- Dharmacon product catalog numbers
- Review the results for accuracy and product availability
- Select the product lines and product formats to include on the plates
- Configure the library plates for quantity, controls, replicates and layout
- Add to cart and check out!
Use the Library Plater to order these products in 96- or 384-well plates
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| Product lines |
siRNA: ON-TARGETplus, siGENOME, Accell and Lincode; microRNA: miRIDIAN mimics and inhibitors; Edit-R CRISPR (KO): crRNA and synthetic sgRNA; CRISPRmod CRISPRa: crRNA; CRISPRmod CRISPRi: synthetic sgRNA. RNAi and CRISPR reagents cannot be mixed together in the same library. |
| Product formats |
siRNA: SMART pool or up to 4 individual siRNAs; microRNA: Mimics or Hairpin Inhibitors; Edit-R CRISPR (KO): Pool or up to 5 individual crRNA and Pool or up to 3 individual synthetic sgRNA; CRISPRmod CRISPRa: Pool or up to 4 individual crRNA; CRISPRmod CRISPRi: Pool or up to 3 individual synthetic sgRNA. |
| Species |
Human or Mouse |
| Plate type |
96 well plates (20 well minimum) or 384 well plates (40 well minimum) |
| Quantity per well |
0.1, 0.25, 0.5, 1 or 2 nmol (1 or 2 nmol per well only available for 96-well plates) |
These options are available but cannot be ordered online. They require a custom quote
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| Labcyte Echo-qualified 384-well plates |
Part# PP-0200. LDV plates not available. |
Which siRNA is right for you?
Horizon offers four complete pre-designed product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.
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Cost-effective, efficient silencing |
High specificity for reduced off-targets + efficient silencing |
Highly specific knockdown of long noncoding RNA (lncRNA) |
Target silencing in difficult-to-transfect cells |
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siGENOME siRNA |
ON-TARGETplus siRNA |
Lincode siRNA |
Accell siRNA |
| Pre-designed for Human, Mouse and Rat protein-coding genes |
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| Pre-designed for Human and Mouse long noncoding RNA (lncRNA) |
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| Recommended for transfectable mammalian cells in culture |
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| Recommended for neuronal, suspension, primary and other difficult-to-transfect cells |
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| Recommended transfection reagent |
DharmaFECT transfection reagent |
DharmaFECT transfection reagent |
DharmaFECT transfection reagent |
None required |
| Available as SMARTpool reagent |
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| Available as four individual siRNAs |
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| Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs |
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| Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake |
Selective application when thermodynamic analysis indicates it necessary for favorable antisense RISC loading |
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| Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity |
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| Modifications to facilitate cellular uptake without separate transfection reagents |
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| Stabilizing modifications to prevent nuclease-mediated degradation |
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| Sequence information provided with purchase |
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SMARTpool siRNA reagent
A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus SMARTpool are guaranteed† to silence by 75% or better.
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Individual siRNA
Select 1, 2, 3 or 4 individual siRNAs† per gene.
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microRNA Mimics and Hairpin Inhibitors
Available for human and mouse microRNAs in miRBase v.21
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Pool of crRNA or sgRNA
A mixture of 4 Edit-R CRISPR (KO) crRNA provided as a single reagent.
A mixture of 4 CRISPRmod CRISPRa synthetic crRNA provided as a single reagent.
A mixture of 3 Edit-R CRISPR (KO) synthetic sgRNA provided as a single reagent.
A mixture of 3 CRISPRmod CRISPRi synthetic sgRNA provided as a single reagent.
May not be combined with RNAi products on the same plate.
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Individual crRNA or sgRNA
Select up to 5 individual Edit-R CRISPR (KO) crRNA per gene.
Select up to 4 individual CRISPRmod CRISPRa synthetic crRNA per gene.
Select up to 3 individual Edit-R CRISPR (KO) synthetic sgRNA per gene.
Select up to 3 individual CRISPRmod CRISPRi synthetic sgRNA per gene.
May not be combined with RNAi products on the same plate.
†Our siRNA knockdown guarantee:
siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100nM siRNA).
Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25nM working concentration.
