CRISPR Screening

Knockout thousands of genes - All at once

The discovery of CRISPR-Cas9 genome editing has provided a novel and highly efficient way to probe gene function through the generation of gene knockouts.

Our CRISPR Screening Service

Horizon offers comprehensive CRISPR screening service using a high performance vector system. The new all-in one vector system offers significantly improved performance in a rapid workflow. Our optimized CRISPR-Cas9 screening platform, sophisticated bioinformatics pipeline and multiple off-the-shelf and custom libraries allow us to design the most appropriate workflow for your screening project to provide you with data of exceptional quality.

Learn how CRISPR-Cas9 screening is going to revolutionize drug discovery

Use our CRISPR screening service to:

  • Identify and prioritize drug targets
  • Find genes required for cell viability, drug sensitivity or resistance
  • Support patient stratification
  • Identify targets or pathways for potential combination therapies

CRISPR Screening Platform

Horizon can provide complete workflows for CRISPR-Cas9 knockout screens, from cell line selection and optimisation through to bioinformatics analysis of the screen results.

CRISPR Screening

Turnaround time: 14-20 weeks

Deliverables: Data in raw and analysed form and a final report containing experimental details and hit nominations

Select one of our off-the shelf sgRNA libraries or have us design a custom library!

  • Cell surface, Signalling & Metabolic
  • Deubiquitinases & Drug Targets
  • Kinases & DNA Damage Response
  • Epigenetics & Autophagy
  • GeCKOv2 whole genome library

Request more information Learn more about our ResponderSCREEN platform

Case study: Whole genome CRISPR-Cas9 resistance screen in A375 melanoma cells

Purpose: A proof of concept study to identify resistance factors against a BRAF kinase inhibitor Vemurafenib (PLX-4032) in A375 melanoma cells that carry a BRAF V600E gain-of-function mutation.

Methods:

  • GeCKO v2 knockout library: 6 sgRNAs against 19,050 genes (Sanjana et al., 2014)
  • Pooled-based approach: lentivirus transduction of the library into cells with antibiotic selection
  • Treatment of the edited cell population with Vemurafenib for 14 days
  • Cell pellet collection and sample preparation
  • Next-generation sequencing and data analysis using an adapted MAGeCK workflow

Results:

  • Highest ranking genes were MED12, NF1, CUL3, NF2, TADA2B and TADA1, which are known to  confer resistance to Vemurafenib.
  • Additional hits include members of the STAGA histone acetyl transferase complex (TAFL5/PAF65b) and the Mediator complex (MED23).

CRISPR Cas9 screen in A375 melanoma cells

 Figure 1. Ranking of the hits of the screen by
MAGeCK algorithm 

 

 

 

Download the complete application note

Seven must-read CRISPR screening papers

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