Research Reagents

The acquisition of Dharmacon has expanded the Horizon Discovery portfolio to include a market-leading suite of research reagents for all aspects of gene modulation for functional analysis, including RNAi, CRISPR-Cas9, and CRISPR activation. Browse this page to browse to your product area of interest.

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CRISPR-Cas9 Gene Editing

Optimized tools for high-confidence gene knockout.

The Edit-R™ CRISPR-Cas9 portfolio enables a wide range of applications for gene knockout, cell engineering, functional screening, and HDR.

  • Ready-to-use lentiviral and synthetic guide RNA reagents predesigned with a validated algorithm for guaranteed target cleavage
  • Ensure robust Cas9 expression with a selection of inducible or constitutive promoters, or explore protein options
  • Empower high-throughput gene editing studies with pooled lentiviral sgRNA or arrayed crRNA libraries

CRISPR Cas9 reagents

CRISPRa for Transcriptional Activation

Synthetic & lentiviral reagents for endogenous gene overexpression

Edit-R CRISPR activation (CRISPRa) reagents provide a straightforward, efficient set of tools to study a gene’s function by overexpression in its native context by 5 to 50,000+ fold!

  • Ready-to-use lentiviral and synthetic guide RNA reagents available for nearly all human and mouse genes
  • Lentiviral and plasmid dCas9-VPR reagents to activate endogenous transcription
  • Arrayed CRISPRa crRNA libraries enable powerful gain-of-function genomic screens

CRISPRa for transcriptional activation

RNA Interference

Guaranteed silencing for high-quality, reproducible results

Dharmacon RNAi products encompass the most complete portfolio of innovative tools for transient, long-term, inducible and in vivo RNAi applications.

  • Predesigned genome-wide siRNA and shRNA reagents for guaranteed silencing and premium specificity
  • Best-in-class microRNA modulation tools for investigation of these regulatory RNAs
  • The widest selection of RNAi screening collections. Choose from whole genome, gene families, or custom libraries


Custom RNA Synthesis

Unparalleled length, yield, and modification options to suit your needs

The innovative properties of Dharmacon 2´-ACE™ technology enable routine synthesis of RNA in high yield and of exceptional quality, higher purity, and greater yields compared to other synthetic methods.

  • RNA molecules with a wide variety of chemical modifications, including dye labeling, non-standard and custom modifications
  • RNA oligos from 2 to 130 bases in length from nmol to gram amounts
  • Custom amidite generation and custom projects to fit your needs

Custom RNA synthesis

Gene Expression

Ectopic gene expression for gene analysis and protein science

Pre-cloned cDNAs and ORFs reduce the amount of time needed to clone or synthesize a gene of interest. Choose from individual clones, lentiviral ORFs, and genome-scale libraries for mammalian species and model organisms.

cDNA and ORF collections

Transfection Reagents

Specialized reagents for high-efficiency delivery of RNA, DNA, and protein

One of the key success factors in any gene modulation experiment (RNAi, overexpression, gene editing) is the introduction of RNA and/or DNA components into your cell line or in vivo system.

  • Multiple DharmaFECT transfection reagents with demonstrated success in extensive cell lines
  • High efficiency of delivery and low cellular toxicity
  • DharmaFECT Duo is the only optimized reagent to deliver multiple RNAs ideal for CRISPR-Cas9 experiments

Transfection Reagents

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