EGFR Multiplex cfDNA Reference Standard Set

Format
cfDNA

Description

The EGFR Multiplex cfDNA Reference Standard Set is a cell line-derived, clinically relevant control that can be used to assess the performance of cfDNA assays that detect somatic resistance mutations in EGFR.

With this product, you are able to:
• Analyze the sensitivity and specificity of your assay
• Gain certainty of the limit of detection and limit of quantification
• Optimize and validate new EGFR-specific assays and routinely monitor their performance

HD825

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Technical Information

High-throughput platforms such as PCR-based assays and Next Generation Sequencing (NGS) give laboratories much greater power when profiling cfDNA samples. However, small quantities of DNA in combination with complex variants at low allelic frequencies represents one of the major challenges users encounter when profiling cfDNA.

Horizon Discovery has developed a comprehensive cfDNA Reference Standard range to support the development and continued validation of cfDNA assays. Our cfDNA products are derived from human cell lines and fragmented to an average size of 160 bp to closely resemble cfDNA extracted from human plasma.

The EGFR Multiplex cfDNA Reference Standard is supplied at 5%, 1%, 0.1% and 0% (EGFR Multiplex wild type) allelic frequencies and covers ten EGFR variants implicated in the responsiveness to EGFR tyrosine kinase inhibitors (EGFR-TKIs) and anti-EGFR monoclonal antibodies. This product covers clinically relevant SNPs, insertions and deletions in EGFR and can be used to optimize, validate or routinely monitor assay performance.

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Technical Data

Genes Covered
EGFR
Allelic Frequencies
5%, 1%, 0.1% and 0% (EGFR Multiplex wild type)
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

Product Information

Fragment Size
160 bp
Unit Size
350 ng per vial
Concentration
20 ng/µl
Verified Mutations
EGFR Multiplex wild type cfDNA Reference Standard: HD824
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.0%
7p12EGFRΔE746 - A7500.0%
7p12EGFRT790M0.0%
7p12EGFRV769 - D770insASV0.0%
7p12EGFRL861Q0.0%
7p12EGFRG719S0.0%
7p12EGFRC797S0.0%
7p12EGFRS464L0.0%
7p12EGFRG465R0.0%
7p12EGFRS768I0.0%
5% EGFR Multiplex cfDNA Reference Standard: HD821
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R5.0%
7p12EGFRΔE746 - A7505.0%
7p12EGFRT790M5.0%
7p12EGFRV769 - D770insASV5.0%
7p12EGFRL861Q5.0%
7p12EGFRG719S5.0%
7p12EGFRC797S5.0%
7p12EGFRS464L5.0%
7p12EGFRG465R5.0%
7p12EGFRS768I5.0%
1% EGFR Multiplex cfDNA Reference Standard: HD822
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R1.0%
7p12EGFRΔE746 - A7501.0%
7p12EGFRT790M1.0%
7p12EGFRV769 - D770insASV1.0%
7p12EGFRL861Q1.0%
7p12EGFRG719S1.0%
7p12EGFRC797S1.0%
7p12EGFRS464L1.0%
7p12EGFRG465R1.0%
7p12EGFRS768I1.0%
0.1% EGFR Multiplex cfDNA Reference Standard: HD823
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.1%
7p12EGFRΔE746 - A7500.1%
7p12EGFRT790M0.1%
7p12EGFRV769 - D770insASV0.1%
7p12EGFRL861Q0.1%
7p12EGFRG719S0.1%
7p12EGFRC797S0.1%
7p12EGFRS464L0.1%
7p12EGFRG465R0.1%
7p12EGFRS768I0.1%
Presence confirmed in parental cell line
EGFR Q787Q, EGFR L844L

General Information

Storage
4°C
Expiry
12 months from the date of manufacture

Quality Control

Fragmentation Size
D1000 DNA Screen Tape assay
Allelic Frequency
Droplet Digital™ PCR
Quantification
Qubit dsDNA BR Assay (post-fragmentation)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 28695
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Batch ID: 35653
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Batch ID: 38666
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Documents & Related Literature

Product Insert
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Product Specification
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Safety Data Sheet
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Order & Delivery Information
If you want to place an order, please contact our distributor
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