BRCA Somatic Multiplex I FFPE

Format
FFPE

Description
The BRCA Somatic Multiplex 1 Reference Standard in FFPE format is a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations. With this product you are able to: • Evaluate workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics • Analyze sensitivity and specificity of your assay • Optimize and validate new cancer panels and routinely monitor the performance of your assay.
HD810
$105.00

Availability: In stock

Dispatched within 24 hours

Bulk order discount
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Technical Information
Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The BRCA Somatic Multiplex 1 portfolio covers multiple endogenous SNPs, including those in difficult to sequence homopolymer regions. The BRCA Somatic Multiplex 1 Reference Standard includes 16 mutations at 7.5% - 100% allelic frequency in genomic DNA and FFPE format. The multiple formats available allow you to validate both the pre-analytical and analytical portion of your workflow using an isogenic reference standard. The FFPE format BRCA Somatic Multiplex 1 reference standard allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples. In addition, the robustness of your NGS workflow may be tested to ensure that your library protocol and informatics pipeline can accommodate degraded FFPE samples. The BRCA Somatic Multiplex 1 reference standard provides a large range of allelic frequencies in 16 onco-relevant mutantations for use across multiple BRCA NGS gene panels.

Technical Data

Genes Covered
BRCA1, BRCA2, BRIP1, BARD1, NBN
Cosmics
16
Fixation Method
4% Formalin
Section Size
15µm or 20µm
Cell Density
3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield
≥ 400 ng using Promega Maxwell LEV Plus Extraction kit

Product Information

Presence confirmed in parental cell line
ChromosomeGenomic CoordinatesGeneVariantExpected Allelic Frequency, %Observed by Targeted Sequencing
1741223094BRCA1S1613G7.55.4
1741244000BRCA1K1183R7.57.1
1741245090BRCA1K820E7.55
1741234451BRCA1R1443STOP32.523.5
1741246245BRCA1D435Y7.56.4
1741244936BRCA1P871L1513.6
1332906480BRCA2N289H7.56.4
1332929387BRCA2V2466A100100
1332911463BRCA2N991D7.54.7
1332913558BRCA2K1691fs32.528.7
1332913836BRCA2N1784fs4033.8
1332912750BRCA2D1420Y32.527.9
1332937354BRCA2I2675fs108.6
1759763347BRIP1S919P100100
2215645464BARD1R378S42.5Not Covered
890990479NBNE185Q60Not Covered
Unit Size
1 FFPE Section
Extractable DNA
≥ 400 ng per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit
Intended Use
For routine performance monitoring (Research Use Only)

General Information

Allelic Frequency
7.5%-100%
Storage
4˚C
Expiry
48 months from the date of manufacture

Quality Control

Genotype
Sanger sequencing of locus specific PCR
Quality
Agarose gel electrophoresis
Quantification
Quantifluor™
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: FFPE-DNA
PDF Download
Batch ID: 34312
PDF Download

Documents & Related Literature

Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

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Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

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If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.

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