BRAF V600E IHC Reference Standard

Synonyms
NS7; BRAF1; RAFB1; B-RAF1

Format Type
Biomarker Specific

Format
FFPE Block

Description
The BRAFV600E Mutation IHC Reference Standard is a highly-characterized quality control material which contains precisely defined positive and negative protein expressing cell lines on the same slide. The BRAFV600E Reference Standard can be utilized during assay development, for optimization, validation of your Laboratory Developed Test (LDT) and verification of a commercial IHC assay. FFPE block format allows for authentic in-process control from sectioning to reporting.
Catalog ID
Qty
Unit Price
HD737
$3,500.00
Bulk order discount

Availability: In stock

Dispatched within 24 hours

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Technical Information
The availability of precisely defined reference materials offers an industry standard for development and quality control of IHC assays, improving the accuracy and reproducibility. IHC HDx Reference Standards provide laboratories with a consistent source of positive and negative reference material on one slide.

Features
• Precisely defined positive and negative protein expressing cell lines on the same slide.
• Cost effective for routine adoption.
• Up to 500 sections (3-5µm) available from a 0.5cm block.
• Cell lines verified by IHC assay and Quantitative Digital Pathology (QDP).

Applications
• Place your sample alongside HDx Reference Standards to provide confidence through verification and validation activities.
• Guide the evaluation of antibodies based on their sensitivity and specificity.
• Gain additional confidence when optimizing and monitoring the performance of your assay through application of a consistent reference standard.

Technical Data

Qualification
Protein expression is confirmed using a biomarker specific IHC assay to ensure the quality of our IHC Reference Standard.
Mutation Type
Mutation
Fixation Method
10% Formalin
Core Diameter
2mm
Cell Density
1-1.5 x 104 cells/core dependent on cell size

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
One 0.5cm FFPE Block

General Information

Storage
4°C
Expiry
24 months

Characterization

Core 1
Core 2
Core 3
Core Legends
Typical Staining pattern of BRAF V600E

Core 1: No cytoplasmic and membrane staining pattern is observed in the negative core
Core 2: Intermediate cytoplasmic and membrane staining pattern is observed in the positive (middle) core
Core 3: Strong cytoplasmic and membrane staining pattern is observed in the positive (right) core

(Spring Bioscience, VE1, #E19292 on the automated Leica BOND Max stainer)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 13958
PDF Download
Batch ID: 19219
PDF Download

Documents & Related Literature

Product Specification
PDF Download
Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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