Recombination Activation Genes (Rag) encode enzymes that play an important role in the rearrangement and recombination of the genes of immunoglobulin and T cell receptor molecules during the process of V(D)J recombination. Rag1 knockout rats lack mature B and T lymphocytes.
• 29 bp deletion within Exon 2 on chromosome 3 • Homozygous Rag1 knockout rats display loss of RAG1 protein via Western blot • Homozygous Rag1 knockout rats show loss of B and T cells by FACS analysis • Background Strain: Sprague-Dawley
Figure 1: Flow cytometric analysis of T cell subsets (CD4 and CD8), B cells and NK cells from wild type and Rag1 (-/-) knockout blood samples The top panels represent wild type female and male rats. The lower panels represent samples from female and male Rag1 (-/-) knockout rats. Blood samples were collected from 4-, 10- and 20-week-old rats.