OncoSpan Reference Standard

Format
Genomic DNA

Description

OncoSpan is the largest and most extensive oncology Reference Standard to date, featuring 386 variants across 152 key cancer genes.

In an exciting new first for Horizon, OncoSpan is provided with batch-specific NGS data, giving you knowledge and confidence of the background genotype of this cell-line-derived Reference Standard. OncoSpan’s larger gene list and better characterisation empowers users to perform more comprehensive validation of large oncology gene panels and exome sequencing assays than ever before.


More information
HD827
$510.00

Availability: In stock

Dispatched within 24 hours

Bulk order discount
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Technical Information

OncoSpan is a well-characterised, cell line-derived Reference Standard containing 386 variants across 152 key cancer genes. This includes 249 variants with a COSMIC ID and 30 INDELs (24 deletions and 6 insertions, ranging from 2-16 base pairs). Variants are present between 1-100% allelic frequency (AF), with 52 variants present at ≤ 20% AF for LOD determination of your assay. Every batch of OncoSpan DNA has 25 variants confirmed by ddPCR, in addition to being fully exome sequenced by GATC-Eurofins at 500x coverage using Agilent SureSelect Human All Exon V6 kit and Illumina sequencing to ISO 17025. This provides you with an accurate and reliable truth set for comparison to your assay’s performance. You will receive a password upon ordering to download the raw NGS data for your batch (bam, bed and vcf files) via our File Transfer Protocol (FTP) portal. Alternatively, for your convenience we have also listed the 386 high confidence variants in an easy-to-access excel document that can be downloaded from the “More Information” button above.


Technical Data

Genes Covered
ABL1, AKT1, AKT2, ALK, APC, AR, ARID1A, ATR, ATRX, AXL, BARD1, BCL6, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BTK, BUB1B, CARD11, CCND1, CCND3, CCNE1, CD79B, CDH1, CDK12, CDK4, CEP57, CFH, CREBBP, CSF1R, CTNNB1, DDR2, DIS3L2, DNMT3A, EGFR, EML4, EP300, EPCAM, ERBB2, ERBB3, ERCC1, ERCC2, ERCC4, ERCC5, ERG, ETS1, ETV4, EWSR1, EXT1, FANCA, FANCD2, FANCE, FANCG, FANCI, FANCM, FBXW7, FGF10, FGF2, FGF3, FGF6, FGFR1, FGFR3, FLCN, FLI1, FLT1, FLT3, FZR1, GATA2, GATA3, GEN1, GNA11, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK1, JAK2, JAK3, KDR, KIT, KRAS, LDLR, MAGI1, MAP2K1, MAP2K2, MAX, MDM4, MED12, MET, MLH1, MLLT3, MMAB, MRE11, MSH2, MSH3, MSH6, MTOR, NBN, NF1, NFE2L2, MOTCH1, NOTCH2, NOTCH3, NRAS, NRG1, NTRK1, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PMS2, PPARG, PPP2R2A, PRKAR1A, PROC, PTCH1, PTPN11, RAD51B, RAD54L, RAF1, RB1, RBM45, RECQL4, RET, RHBDF2, ROS1, RPS6KB1, SDHB, SF3B1, SF3B2, SLTM, SLX4, SMARCB1, SMO, SMOX, STK11, TERT, TET2, TFRC, TP53, TP53BP1, TSC1, TSC2, WRN, XPA, XPC, ZNF395
Cosmics
249
Allelic Frequencies
1% - 92.5% for ddPCR-verified variants
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

Product Information

Verified Mutations
ChromosomeGeneVariantExpected Allelic Frequency %
chr2 (29416025)ALKN/A (Ins)10.0%
chr5 (112175770)APCp.T1493T35.0%
chr7 (140453136)BRAFp.V600E10.5%
chr13 (32913558)BRCA2p.K1691fs*1532.5%
chr3 (41266101)CTNNB1p.S33Y32.5%
chr3 (41266134)CTNNB1p.S45del10.0%
chr7 (55241707)EGFRp.G719S24.5%
chr7 (55249071)EGFRp.T790M1.0%
chr7 (55259515)EGFRp.L858R3.0%
chr7 (55242465)EGFRp.E746_A750 delELREA2.0%
chr7 (55249063)EGFRp.Q787Q15.0%
chr4 (153244156)FBXW7p.S668fs*3932.5%
chr13 (28578215)FLT3p.P986fs*>810.0%
chr4 (55599321)KITp.D816V10.0%
chr4 (55602765)KITp.L862L7.5%
chr12 (25398281)KRASp.G13D15.0%
chr12 (25398284)KRASp.G12D6.0%
chr7 (116436022)METp.A1357A7.0%
chr7 (116339848)METp.L238fs*257.0%
chr9 (139409754)NOTCH1p.P668S30.0%
chr1 (115256530)NRASp.Q61K12.5%
chr3 (178936091)PIK3CAp.E545K9.0%
chr3 (178952085)PIK3CAp.H1047R17.5%
chr10 (43613843)RETp.L769L60.0%
chr17 (7579472)TP53p.P72R92.5%
Unit Size
1µg
Concentration
50ng/µl

General Information

Storage
4˚C
Expiry
36 months from the date of manufacture

Quality Control

Allelic Frequency
Droplet Digital PCR™
Genotype
Next Generation Sequencing
Quality
Agarose gel electrophoresis
Quantification
Spectrophotometry (A260)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: gDNA-multiplex
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

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