OncoSpan is a well-characterised, cell line-derived Reference Standard containing 386 variants across 152 key cancer genes. This includes 249 variants with a COSMIC ID and 30 INDELs (24 deletions and 6 insertions, ranging from 2-16 base pairs). Variants are present between 1-100% allelic frequency (AF), with 52 variants present at ≤ 20% AF for LOD determination of your assay. Every batch of OncoSpan DNA has 25 variants confirmed by ddPCR, in addition to being fully exome sequenced by GATC-Eurofins at 500x coverage using Agilent SureSelect Human All Exon V6 kit and Illumina sequencing to ISO 17025. This provides you with an accurate and reliable truth set for comparison to your assay’s performance. You will receive a password upon ordering to download the raw NGS data for your batch (bam, bed and vcf files) via our File Transfer Protocol (FTP) portal. Alternatively, for your convenience we have also listed the 386 high confidence variants in an easy-to-access excel document that can be downloaded from the “More Information” button above.
|Chromosome||Gene||Variant||Expected Allelic Frequency %|
|chr2 (29416025)||ALK||N/A (Ins)||10.0%|
|chr7 (55242465)||EGFR||p.E746_A750 delELREA||2.0%|
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