OncoSpan Reference Standard

Format
Genomic DNA

Description

OncoSpan is the largest and most extensive cell line-derived Reference Standard to date, featuring 386 variants across 152 key cancer genes.

OncoSpan is provided with batch-specific NGS data and a companion NGS QC tool called OncoMatic, allowing the most comprehensive validation of oncology gene panels and exome sequencing assays.


Find NGS Variants here

5% discount on online orders

Use 'ONLINE5'. *Terms apply

 
Catalog ID
Qty
Unit Price
HD827
$510.00
Bulk order discount

Availability: In stock

Dispatched within 24 hours

Total:
OR
Technical Information

OncoSpan is a well-characterised, cell line-derived Reference Standard featuring 386 variants across 152 key cancer genes. This is the largest number of genes featured in any commercial Reference Standard to date, allowing the most comprehensive validation of oncology gene panels and exome sequencing assays. The background genome of this Reference Standard has been characterised by high coverage exome sequencing, and in a progressive step forward, OncoSpan is now provided with batch-specific NGS data available to every customer. This empowers users with access to the most accurate Reference Standard truth set for use during the establishment and validation of critical bioinformatics pipelines.

OncoSpan has been designed to work alongside the top NGS gene panels to streamline oncology genetics and facilitate effective integration of highly validated and trusted NGS assays. During this process, the 386 variants in OncoSpan have been cross-checked against gene panel amplicon design of Thermo Fisher’s Oncomine and Illumina’s TruSight gene panels.

OncoSpan is supported by OncoMatic, a bespoke NGS quality control (QC) tool. OncoMatic allows you to upload your NGS data after sequencing OncoSpan DNA and automatically calculates key metrics to assess sensitivity and specificity of your assay. The software allows users to easily track Reference Standard performance over time, making routine QC management more efficient and convenient, giving you effective control over your data.


Technical Data

Genes Covered
ABL1, AKT1, AKT2, ALK, APC, AR, ARID1A, ATR, ATRX, AXL, BARD1, BCL6, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BTK, BUB1B, CARD11, CCND1, CCND3, CCNE1, CD79B, CDH1, CDK12, CDK4, CEP57, CFH, CREBBP, CSF1R, CTNNB1, DDR2, DIS3L2, DNMT3A, EGFR, EML4, EP300, EPCAM, ERBB2, ERBB3, ERCC1, ERCC2, ERCC4, ERCC5, ERG, ETS1, ETV4, EWSR1, EXT1, FANCA, FANCD2, FANCE, FANCG, FANCI, FANCM, FBXW7, FGF10, FGF2, FGF3, FGF6, FGFR1, FGFR3, FLCN, FLI1, FLT1, FLT3, FZR1, GATA2, GATA3, GEN1, GNA11, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK1, JAK2, JAK3, KDR, KIT, KRAS, LDLR, MAGI1, MAP2K1, MAP2K2, MAX, MDM4, MED12, MET, MLH1, MLLT3, MMAB, MRE11, MSH2, MSH3, MSH6, MTOR, NBN, NF1, NFE2L2, MOTCH1, NOTCH2, NOTCH3, NRAS, NRG1, NTRK1, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PMS2, PPARG, PPP2R2A, PRKAR1A, PROC, PTCH1, PTPN11, RAD51B, RAD54L, RAF1, RB1, RBM45, RECQL4, RET, RHBDF2, ROS1, RPS6KB1, SDHB, SF3B1, SF3B2, SLTM, SLX4, SMARCB1, SMO, SMOX, STK11, TERT, TET2, TFRC, TP53, TP53BP1, TSC1, TSC2, WRN, XPA, XPC, ZNF395
Cosmics
195
Allelic Frequencies
1% - 92.8% for ddPCR-verified variants
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

Product Information

Verified Mutations
ChromosomeGeneVariantExpected Allelic Frequency %
chr2 (29416025)ALKN/A (Ins)10.0%
chr5 (112175770)APCp.T1493T35.0%
chr7 (140453136)BRAFp.V600E10.5%
chr13 (32913558)BRCA2p.K1691fs*1532.5%
chr3 (41266101)CTNNB1p.S33Y32.5%
chr3 (41266134)CTNNB1p.S45del10.0%
chr7 (55241707)EGFRp.G719S24.5%
chr7 (55249071)EGFRp.T790M1.0%
chr7 (55259515)EGFRp.L858R3.0%
chr7 (55242465)EGFRp.E746_A750 delELREA2.0%
chr7 (55249063)EGFRp.Q787Q15.0%
chr4 (153244156)FBXW7p.S668fs*3932.5%
chr13 (28578215)FLT3p.P986fs*>810.0%
chr4 (55599321)KITp.D816V10.0%
chr4 (55602765)KITp.L862L7.5%
chr12 (25398281)KRASp.G13D15.0%
chr12 (25398284)KRASp.G12D6.0%
chr7 (116436022)METp.A1357A7.0%
chr7 (116339848)METp.L238fs*257.0%
chr9 (139409754)NOTCH1p.P668S30.0%
chr1 (115256530)NRASp.Q61K12.5%
chr3 (178936091)PIK3CAp.E545K9.0%
chr3 (178952085)PIK3CAp.H1047R17.5%
chr10 (43613843)RETp.L769L60.0%
chr17 (7579472)TP53p.P72R92.5%
Unit Size
1µg
Concentration
50ng/µl

General Information

Storage
4˚C
Expiry
36 months from the date of manufacture

Quality Control

Allelic Frequency
Droplet Digital PCR™
Genotype
Next Generation Sequencing
Quality
Agarose gel electrophoresis
Quantification
Spectrophotometry (A260)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: gDNA-multiplex
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

Back to top