Interested in a complete study? Horizon now offers our In Vivo Drug Transporter Assay service. P-glycoprotein plays a critical role in efflux for both brain and liver. Homozygous null Mdr1a rats display increased exposure to CNS drugs in the brain, as well as increased bioavailability in the plasma for P-gp-specific substrates.
• Biallelic 20 bp deletion within Abcba1 gene • Increased oral bioavailability of P-gp-specific substrates • Homozygous knockout rats display total loss of protein via Western blot • Background Strain: Sprague-Dawley
Figure 1: Oral absorption of Digoxin in the absence and presence of Quinidine
Figure 2. Western blot using proximal colon tissue isolated from both wild type and Mdr1a homozygous knockout tissue