Microinjection Ready Reagents

Microinjection Ready Reagents

Customize your own animal models

Overview

If you want to generate your own in vivo models, Horizon can support you. We provide access to professional in vivo-grade, microinjection ready CRISPR reagents expertly designed by the leaders in genetic engineering.

We offer three levels of microinjection, in vivo-grade CRISPR-Cas9 reagents.

Basic

Microinjection-ready reagents. Basic service pricing

5 aliquots
sgRNA with Cas9 Protein

 
 

 
 

 
 

Validated

Microinjection-ready reagents. Validated service pricing

5 aliquots
sgRNA with Cas9 Protein

Design of sgRNA
specific for your project goals

In vitro validation
of cutting activity

Genotyping protocol
and validated primers

Validated Plus

Microinjection-ready reagents. Validated Plus service pricing

5 aliquots
sgRNA with Cas9 Protein

Design of sgRNA
specific for your project goals

In vitro validation
of cutting activity

Genotyping protocol
and validated primers

Off target assay
primers for off target sites

* Prices shown are only for the sgRNA and Cas9 Protein, knock-in projects requiring a donor or additional sgRNAs will result in an increase of price.

Licensed by Caibou Biosciences and the Broad Institute.

Basic

Our Microinjection-Ready Basic CRISPR-Cas9 reagents include an sgRNA targeting your gene (for knockout applications) and Cas9 protein, and come packaged separately to allow for concentration optimization.

Included:Price:
  • 1 aliquot sgRNA for your target gene
  • 1 aliquot Cas9, supplied as protein
  • 1 aliquot Microinjection Buffer
  • $990 (academic)/ $1,490 (industry)

Validated

At Horizon, we validate all of our CRISPR-Cas9 reagents for cutting efficiency before we inject. With our validated reagents, we'll send you only reagents that are proven to cut with high efficiency in an in vitro assay. We'll design five sgRNAs and will send you the best performer.

You'll also have access to our experienced design team. Our design team has designed nucleases for hundreds of mouse, rat, and even rabbit projects, and has a deep understanding of the optimum target sites for every application. They'll expertly design your sgRNAs for your specific project and application.

Included:Price:
  • Design service-project-specific sgRNA design by our experienced scientists
  • In vitro validation of sgRNA cutting activity
  • 1 aliquot each of sgRNA for your target gene, Cas9 Protein, and Microinjection Buffer
  • Genotyping protocol and primer sequences
  • $1,990 (academic)/ $2,490 (industry)

Validated Plus

Our Validated+ reagents are our most complete package and offer the most confidence as you generate your model. You'll receive our same expert design and validation services along with a detailed genotyping protocol with validated primers specifically designed for your project. You'll also receive validated primers for the top 10 assayable potential off-target sites for your project. These primers will allow you to demonstrate the specificity of your CRISPR-Cas9 reagents and ease concerns regarding potential off-target effects.

Included:Price:
  • Design service-project-specific sgRNA design by our experienced scientists
  • In vitro validation of sgRNA cutting activity
  • 1 aliquot each of sgRNA for your target gene, Cas9 Protein, and Microinjection Buffer
  • Validated genotyping primers and protocol
  • Validated primers for the top 10 assayable potential off-target sites
  • $3,990 (academic)/ $4,490 (industry)

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