Human BRAF (V600E/+) MCF10A Cell Line

B-Raf, B-RAF1, BRAF1, NS7, RAFB1

Heterozygous knockin of BRAF activating mutation (V600E), Pre-Cre recombinase
Catalog ID
Unit Price
HD 101-026

Academic pricing applied

Bulk order discount

Availability: In stock

Dispatched within 24 hours


Commercial pricing Please contact us or call 1-844-655-7800

Technical Information

Technical Data

Hugo Name


HD Derivation Slide
Cell Line


Functional Area
MAPK signalling
Related References
Di Nicolantonio, F., et al. (2008). Replacement of normal with mutant alleles in the genome of normal human cells unveils mutation-specific drug responses. PNAS 105(52):20864-20869.
Ready to Ship
<7 days

General Information

Parental ATCC ID
Key Endogenous Mutations
none listed
STR Profile
Amelogenin: X ; CSF1PO: 10,12 ; D13S317: 8,9 ; D16S539: 11,12 ; D5S818: 10,13 ; D7S820: 10,11 ; THO1: 8,9.3 ; TPOX: 9,11 ; vWA: 15,17; ATCC
Homo sapiens

Cell Line Characteristics

Gene ID
Link To NCBI
Genomic Locus
CVT Selection Condition
G418 resistant - 0.3mg/ml

Quality Control

BRAF (V600E/+)
Cell Number

Cell Culture

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 75cm2 flask containing pre-warmed media.
Media Type
DMEM/F-12 including 2.5 mM L-glutamine and 15 mM HEPES, supplemented with 5% horse serum, 10 µg/mL insulin, 20 ng/mL hEGF, 0.5 µg/mL hydrocortisone, 0.1 µg/mL cholera toxin.
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 80-90% confluency, approximately 1:6-1:10.
Biosafety Level
Freeze Medium
Complete growth media 45%; USDA approved FBS 50%; DMSO 5%
Customer FAQs

View our collection of frequently asked questions below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.


What are X-MAN Cell Lines?

Horizon's X-MAN® (Mutant And Normal) cell lines are genetically engineered cell pairs containing defined genomic modifications, providing a model system for the study of the role of genes in cellular processes and drug responses. Cell lines are provided with a matched wild type control parental cell line ensuring phenotypes can be attributed directly to the genotype.

What should I use X-MAN Cell Lines for?

Using Horizon’s X-MAN Cell Lines you can:

  • Study loss or gain of function mutations at the endogenous level
  • Initiate target identification and validation studies
  • Identify responsive or resistant patient populations in a human cell line model
  • Analyse expression, protein interactions and localization using endogenously tagged genes

Are mutant cell lines provided with a wild type control?

Cell lines are provided as isogenic pairs – the modified mutant line and the originating wild type parental line. 

How are X-MAN® Cell Lines validated?

All X-MAN Cell Lines are validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. To enable you to verify our results, we provide our sequencing result, including primer sequences.

How does Horizon confirm X-MAN® cell lines are free from contamination?

We follow strict bio-banking procedures and our cells are regularly tested for contamination, including mycoplasma.

Under what conditions should I store my X-MAN Cell Lines?

Our cell lines are shipped on dry ice but should be stored in standard mammalian cell culture storage conditions using liquid nitrogen for long-term storage.

I have never worked with these cells before.  How do I know if they look correct?

Our Technical Support team is happy to provide a visual inspection of your culture if you send an image to

Do you provide the PCR conditions for your cell line genomic validation?

The primer sequences used for our internal genomic validation are provided on the Certificate of Analysis and Datasheet provided with the cell lines. We do not provide the PCR reaction conditions as these should be optimized within your own laboratory.

What other validations do you recommend to be performed?

The ultimate proof that a cell line does not express the gene of interest is protein expression analysis by Western blot or similar. Unfortunately, due to the costs and time involved in optimising any new antibody,

Horizon cannot test protein expression in all its cell lines. As such we recommend that protein expression is assessed in cell lines prior to proceeding with downstream experiments.

If you would like to learn more about our products then please browse our dedicated Cell Line FAQ page here

Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:


Our online catalog and ordering system can be used to search our full menu of available Cell Lines. Select your desired products and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.


If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
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Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

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Rest of the World
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FEDEX International Priority

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs.

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

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