BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM, Mar 2008].
HAP1 knockout cell lines
The single largest bank of isogenic cell lines with over 7,500 cell lines to choose from and trusted by academia, biotech, and pharma research labs. Cell models to suit any application, from TIDVAL to antibody validation, delivered in as few as 10 days.
Advantages of HAP1 knockout cell lines
- Easily characterize knockout of your gene-of-interest in a biological context
- Ready-made knockout cell lines are available for all non-essential human genes
- Editing has been confirmed by Sanger sequencing of genomic DNA
- Two independent knockout products for each gene, allowing second-clone analysis if more than one data point is required
Proven models
Our catalog of cell lines has become a gold-standard resource
in antibody validation, cancer research, and early drug-discovery research. Horizon was recently recognized by CiteAb as the Cell Line Supplier to Watch in Cancer Research for 2024. Read more about the CiteAb award.
Why are HAP1 cells an ideal option for gene-edited cell line models?
Our HAP1 cell line database represents the synergy of two great systems - the amenability of human haploid HAP1s to gene editing and CRISPR-Cas9 technology. HAP1 is a human near-haploid cell line derived from the chronic myelogenous leukemia (CML) cell line KBM-7. KBM-7 cells were derived from a male patient and lack a Y chromosome. Therefore, haploid HAP1 cells contain a single X chromosome. For more details on how the HAP1 cell line was created and their full genotype, please see the references tab.
HAP1 cell line properties include:
- Sustained growth at single cell dilutions
- Ease of transfection
- Rapid doubling time
- Not selected for ploidy status
- Will have haploid and diploid populations
The majority of commonly used cell lines are diploid or even have more than two copies of any particular allele. When gene editing for loss of function mutations, each allele has to be modified for the phenotype to be expressed. The HAP1 cell line has the advantage of having one copy of each gene meaning you can be sure the edited allele will not be masked by addition alleles. With passaging, HAP1 cells will spontaneously diploidize over time. However, the knockout will not be affected by diploidization as it is a duplication of the edited allele that gives rise to the diploid state.
See our HAP1 publication list to see how the cell line has been applied for a wide range of biological processes and assays, such as DNA damage repair pathway, stress responses, disease modeling as well as antibody validation.
Limited use agreement
All cell lines engineered by Horizon Discovery are purchased under the terms of our Limited Use Label License.