Human ANGPTL4 knockout cell line 4bp deletion

Gene Synonyms
ANGPTL2, ARP4, FIAF, HARP, HFARP, NL2, PGAR, pp1158, UNQ171

Description
HAP1 cell line, edited by CRISPR/Cas to contain a 4bp deletion in a coding exon of ANGPTL4.

This product includes the following:
• Genetically modified cell line
• Matched control
HZGHC006555c009
$1,950.00

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Technical Information

Technical Data

Parental Cell Line
HAP1
Adherent Suspension
Adherent
Gene ID NCBI
51129
Genomic Location
chr19:8366040
Genome Assembly
hg38

General Information

Organism
Homo sapiens
Storage
LN vapor (less than -130°C)

Cell Line Characteristics

HGNC Symbol

ANGPTL4

Expression Level
0.67 TPM
The TPM expression value indicates this gene is
not expressed
Guide RNA Sequence
TTTGCAGATGCTGAATTCGC
Targeted Transcript
NM_001039667
Targeted Region
Exon 2
Mutation
4bp deletion
Transcript Plot Name

Quality Control

PCR FWD
ATGGAGATTTGGAAGGATGGATGAG
PCR BWD
CTCATGGTCTAGGTGCTTGTGGTC
Sequencing Primer
ATGGAGATTTGGAAGGATGGATGAG
Sequencing Result
gGGGTaGGcAGCTGGGTCCTCACCAAGGTTTTCaCCCCTCCCCAGACACAACTCAAGGCTCAGAACAGCAGGATCCAGCAACTCTTCCACAAGGTGGCCCAGCAGCAGCGGCACCTGGAGAAGCAGCACCTGCGCAGCATCTGCAAAGCCAGGTAACCCTAGGATCAAGGGAGAAAAGGTCCCTCTGATAGCTGGACCCCAGGTTGAGAGGGAGGTGGTGAGAACTGGACGTGTGGCTGGGGACGTGGGGCCAGGCAGGACCTGACACCCTCCTCCCGTCCCATCCTAGTTTGGCCTCCTGGACCACAAGCACCTAgnACnATgag
Genotype
ANGPTL4 HAP1 knockout

Cell Culture

Biosafety Level
1
Media Type
IMDM, 10% FCS
Freeze Medium
IMDM, 20% FCS, 10% DMSO
Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.
Growth Properties
Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Protocols & Documents

Documents & Related Literature

Datasheet
PDF Download
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