Human ACD knockout cell line 62bp deletion

Gene Synonyms
PIP1, PTOP, TINT1, TPP1

Description
HAP1 cell line, edited by CRISPR/Cas to contain a 62bp deletion in a coding exon of ACD.

This product includes the following:
• Genetically modified cell line
• Matched control
HZGHC005062c003
$1,950.00

Availability: In stock

Within 7 business days

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Technical Information

Technical Data

Parental Cell Line
HAP1
Adherent Suspension
Adherent
Gene ID NCBI
65057
Genomic Location
chr16:67660359
Genome Assembly
hg38

General Information

Organism
Homo sapiens
Storage
LN vapor (less than -130°C)

Cell Line Characteristics

HGNC Symbol

ACD

Expression Level
42.19 TPM
The TPM expression value indicates this gene is
expressed
Guide RNA Sequence
CGCACGAGGGCGTCCTGCTC
Targeted Transcript
NM_001082486
Targeted Region
Exon 1
Mutation
62bp deletion
Transcript Plot Name

Quality Control

PCR FWD
CAGAATCAGCTCCCGAATCCA
PCR BWD
CTACCGGTACCACAGGAATCTTGA
Sequencing Primer
CAGAATCAGCTCCCGAATCCA
Sequencing Result
tGCcnTCCCcaCGGCTACnCCCAGCGGAtGCAACGGGCCCGGGTTTCCCGCGGGCGCCCAGGCCCCGCCTTTCCTCGGAAGAGGAAGCTCCTTCGCTGGGCGGGGCCGGAGGAGGAGGCCCCGCCCACGTACACCCCGCTGGTGCACGGGATGCTGGCCCGTTTACTCTCATCGCGGCGTCACTCTGACAGCGGCCAGGCATTTGGGCCGTTCGTCAAGATTCCTGTGGTACCGGTAG
Genotype
ACD HAP1 knockout

Cell Culture

Biosafety Level
1
Media Type
IMDM, 10% FCS
Freeze Medium
IMDM, 20% FCS, 10% DMSO
Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.
Growth Properties
Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Protocols & Documents

Documents & Related Literature

Datasheet
PDF Download
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