Apolipoprotein E (ApoE) is a critical apoprotein of the chylomicron which binds to a specific receptor on liver cells and peripheral cells. Additionally, the 4 allele of ApoE (ApoE4) is a major risk factor for Alzheimer's disease (AD), with possession of at least one ApoE4 allele in 40–65% of patients with AD and a patient with 2 ApoE4 alleles having up to 20 times the risk of developing AD.
- Diet Value
LabDiet #5R24 (RMH2500)
- Health Report
- Research Application
• Alzheimer's disease
• Neurodegenerative diseases
- Research Area
• Homozygous replacement of the Rat ApoE gene with the Human ApoE4 gene
• Background Strain: Sprague-Dawley
- Figure 1
Figure 1: Humanized Knock-in Validation of the ApoE4 5' junction, 3' junction, and Donor Cassette via PCR. PCR analysis to show targeted integration of the humanized donor cassette.