Colorectal Cancer Panel FFPE Reference Standard

This Revvity Mimix reference standard is the FFPE version of Colorectal Cancer Panel cfDNA Reference Standard (HD842). This Colorectal Cancer Panel FFPE Reference Standard  mimics patient samples and can be used for end-to-end validation of molecular assays designed for testing colorectal cancer specific mutations.

Colorectal cancers (CRC) are the second leading cause of cancer-related mortality globally as of 2020. In the pursuit of diagnosis and personalized treatment strategies for CRC patients, molecular/genetic profiling plays a pivotal role. As with all the solid tumors, FFPE is the preferred choice of sampling to detect mutations which are diagnostically or prognostically relevant. Our CRC FFPE reference panel is meticulously blended using a unique combination of engineered cell lines harboring clinically significant mutations specific to CRCs. This FFPE panel replicates patient samples and serves as a robust reference material for validating NGS and other molecular biology methodologies aimed at detecting actionable mutations in key genes such as AKT1, APC, BRAF, KRAS, PIK3CA, and TP53 (refer to the accompanying table for a complete gene list). Like all Revvity Mimix reference standards, this product is manufactured in compliance with ISO 9001:2015 and ISO 13485:2016 standards.

Batch-specific supplementary NGS data

The Colorectal Cancer Panel FFPE Reference Standard comes with a batch specific supplementary NGS data. You can access the VCF file here. Each batch was sequenced using Agilent's CREV4 (Clinical Research Exome) kit at a depth of 250x on Illumina platform and run through the Illumina DRAGEN Somatic Pipeline for mapping, sorting, and variant calling.

 

Technical Data

Format: FFPE

Fixation Method: 4% PFA (w/v)

Section Size: 15µm

Cell Density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section

Expected DNA Yield: 400 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit

Genes covered: ABL1, ALK, AKT1, APC, BRAF, PIK3CA, KRAS, NRAS, PTEN, TP53

Allelic Frequencies: 3.00 - 22.00%

Verified Mutations: 

Gene DNA sequence change Amino acid change Expected allelic frequency (%)
ABL1 c.1001C>T p.T315I 5.00
AKT1 c.49G>A p.E17K 3.00
ALK c.3522C>A p.F1174L 5.00
APC c.8140C>T p.R2714C 22.00
BRAF c.1799T>A p.V600E 4.00
KRAS c.35G>A p.G12D 5.00
NRAS c.181C>A p.Q61K 4.00
PIK3CA c.1624G>A p.E542K 5.00
PTEN c.388C>T p.R130* 5.00
TP53 c.722C>T p.S241F 8.00

 

General information

Storage:  4°C

Shipping:  Ambient

Expiration:  See all product shelf life information

Intended use:  For assay developers, molecular diagnostic labs (Research use only)

 

Quality control

Allelic frequency:  Droplet Digital™ PCR

Quantification:  Quantifluor

DNA Integrity:  Agarose gel electrophoresis

Manufacturing Quality:  ISO 9001:2015 and ISO 13485:2016