Colorectal Cancer Panel ctDNA negative control
The Colorectal Cancer ctDNA Panel reference standard negative control is thoroughly tested and confirmed to be negative for all the 10 clinically relevant mutations using ddPCR. This cell line-derived panel is manufactured under ISO 9001:2015 and ISO 13485:2016. This panel can be used as a negative control or to dilute the allele frequency of the CRC ctDNA panel reference standard - HD842, further enabling assessment of MRD.
Technical data
Format: ctDNA
Genes covered: ABL1, ALK, AKT1, APC, BRAF, PIK3CA, KRAS, NRAS, PTEN, TP53
Allelic frequencies: 0.00%
Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Product information
Fragment size: 160 bp
Unit size: 350 ng per vial
Concentration: 20 ng/µl
Verified mutations:
| Gene | DNA sequence change | Amino acid change | Expected allelic frequency (%) |
|---|---|---|---|
| ABL1 | c.1001C>T | p.T315I | 0.00 |
| ALK | c.3522C>A | p.F1174L | 0.00 |
| AKT1 | c.49G>A | p.E17K | 0.00 |
| APC | c.8140C>T | p.R2714C | 0.00 |
| BRAF | c.1799T>A | p.V600E | 0.00 |
| PIK3CA | c.1624G>A | p.E542K | 0.00 |
| KRAS | c.35G>A | p.G12D | 0.00 |
| NRAS | c.181C>A | p.Q61K | 0.00 |
| PTEN | c.388C>T | p.R130* | 0.00 |
| TP53 | c.722C>T | p.S241F | 0.00 |
General information
Storage: 4°C
Expiration: See all product shelf life information
Intended use: For assay developers, molecular diagnostic labs (Research use only)
Quality control
Fragmentation size: D1000 DNA ScreenTape assay
Allelic frequency: Droplet Digital™ PCR
Quantification: Qubit® dsDNA BR assay (Invitrogen)
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Additional Resources
Application notes
Certificate of analysis
Safety data sheets
Selection guides
Reference Standards
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At what temperature do you ship reference standards?
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Can variants in the ctDNA Colorectal Cancer Panel (HD842) be detected with NGS?
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How are Horizon cfDNA Reference Standards different from other circulating tumor DNA controls?
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How are Horizon cfDNA Reference Standards fragmented?
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How do you manufacture your Horizon Reference Standards?
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Is it possible to order custom versions of Horizon Reference Standard?
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Is the cell line background of the ctDNA Colorectal Cancer Panel (HD842) the same as that of the negative control (HD843)?
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What are cfDNA Reference Standards?
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What is MRD?
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What is the difference between ctDNA and cfDNA?
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Why is the ctDNA Colorectal Cancer Panel (HD842) offered at very low allele frequencies?
Related Products
Improve your MRD assay validations using the new Colorectal Cancer Panel ctDNA Reference Standard. It is a well characterized, cell line-derived control material manufactured under ISO 9001:2015 and ISO 13485:2016 that contains 10 clinically relevant variants across 10 genes.