CFTR knockout rats possess a 16 bp deletion in exon 3 of the Cystic Fibrosis transmembrane conductance regulator (CFTR), resulting in loss of protein expression.
LabDiet #5R24 (RMH2500)
• Cystic Fibrosis • Chloride transport • Thiocyanide transport
Figure 1:Proximal nasal histology and nasal potential difference measurements in CFTR knockout rats. (A) Low power magnification (4×) H&E stained sections from the proximal nasal passages bar = 500 µm. 20× images of ABPAS stained nasal septa from boxed areas bar = 25 µm. Arrowheads, cells swollen with intracellular mucus; e, respiratory epithelium; g, submucosal gland; dashed line (–), basement membrane (n = 4 animals/group) (B) NPD tracings from wild-type and CFTR-/- rats. Summary data from NPD measurements for: amiloride, Cl--free Ringers, forksolin, and Cl-/-free Ringers+forskolin. (n = 5 animals/group) **p=0.01. Tuggle KL, et al. (2014) PLoS ONE 9(3): e91253.