Cell lines with endogenously tagged genes enable researchers to track protein production and localisation in real time in live cells. These cell lines are also suitable for western blotting, protein pull down, affinity chromatography, immunocytochemistry and flow cytometry among others. These can also be a good solution when working with a protein for which there is no good antibody.
Turbo GFP has bright green fluorescence visible earlier than fluorescence of other green fluorescent proteins. These properties mean that it is especially useful for live cell applications where fast appearance of bright fluorescence is crucial, such as cell and organelle labelling and tracking the promoter activity.
We have adapted CRISPR CAS9 gene-editing technology to quickly and efficiently tag genes in our proprietary cell line HAP1 with Turbo GFP. This service can deliver projects in 16-18 weeks.
Alternatively, see our new range of cell lines with fluorescent organelles:
This example shows HAP1 cells gene-edited to incorporate Turbo GFP at the endogenous level for LMNA, which is a nuclear membrane protein, and for TERF1 which locates to the telomeres in the nucleus, showing the expected patterns of localisation.
For more information and/or to discuss experimental design:
Read our published article for more details about the methodology behind this product.