Knockin Cell Lines
Recapitulate or even correct disease phenotypes
Disease relevant mutations can be recapitulated or even correct by introducing a point mutation with a single base change, or by inserting/deleting large gene sequences. These endogenous knockin cell lines enable a clear understanding of the contribution of the gene or mutation to a phenotype, in contrast to overexpression models where high expression (either transiently or stably) can lead to false positives. Horizon produces rAAV and CRISPR knockin cell lines or a combination of both.
Use the search bar at the top of the page to browse for your gene of interest, using the HUGO Gene Nomenclature.
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Alternatively, if you can't find your cell line of interest, see our custom cell lines service:
Advantages of Horizon's knockin cell lines:
Isogenic cell lines allow the role of specific genes to be studied without the influence of other factors. A suite of the most common and clinically relevant mutations EGFR mutants [EGFR ∆E746-A750; EGFR L858R, EGFR T790M] were generated. The data below demonstrates the sensitivity profiles of these mutations to the anti-proliferative effects of the EGFR inhibitors gefitinib and erlotinib in 96hr proliferation assays.
Isogenic cell lines allow the role of specific genes to be studied without the influence of other factors. The data above shows the anti-proliferative effects of the EGFR inhibitors gefitinib and erlotinib on cell lines containing different engineered EGFR mutants [EGFR ∆E746-A750; EGFR L858R, EGFR T790M] in 96hr proliferation assays.
Making Knockin Cell Lines
A double-strand break is introduced by targeting the CRISPR-Cas9 to a specific site in the genome, in the presence of a suitably designed homologous donor sequence.
If the repair of that double strand break occurs via the homology-directed repair pathway, the donor sequence can be inserted and a single base change is introduced in to that gene.