Cell Line Engineering

Cell Line Engineering

Custom gene-edited cell lines with your chosen genotype

Overview

With nearly a decade of experience planning and executing nearly 3,000 gene editing experiments, access to a multiple genome editing technologies, and cell line development service options to suit a variety of experimental needs and budgets, Horizon is the global leader in cell line engineering.

Custom Cell Line Generation

Starter
  • Sequence verified biallelic KO* or monoallelic KI via CRISPR-Cas9^#
  • DLD-1, HAP1, HCT-116, or RKO cell line
  • Predesigned sgRNA (KO) or screening of 2 sgRNAs (KI)

From 10 weeks

Standard
  • Sequence verified biallelic KO* or monoallelic KI via CRISPR-Cas9^#
  • Any standard cancer cell line1**
  • Cell line characterization and optimization (if not used before)***
  • Predesigned sgRNA (KO) or screening of 2 sgRNAs (KI)
  • Mycoplasma Hoechst testing

From 13 weeks

Premium
  • Sequence verified biallelic KO* or monoallelic KI via any/all of our gene editing^ technologies
  • Any cell line1
  • Cell line characterization and optimization
  • Custom sgRNA design (KO) or screening of 5 sgRNAs (KI)
  • Mycoplasma Hoechst and culture isolation testing
  • Extended validation

From 20 weeks

*Knockouts by NHEJ-mediated frame-shift mutations. Defined deletions and gene disruption via targeted insertion are subject to knockin pricing. Some cell lines may be polyploid at some loci. Additional alleles may be modified for an additional fee. **Possessed by Horizon or in ATCC for the commercial use. ***Characterization of more than one cell line is available for an additional fee. 1Cell line must tolerate single cell cloning and display adequate transfection efficiency. ^KI of additional alleles is available for an additional fee. #A plasmid donor carrying a selection cassette will be utilized.

Key Benefits
  • Strong IP position to support your freedom to operate
  • Substantial experience with over 100 cell lines and 200 target genes
  • Emphasis on design to test your hypothesis, not just project execution
  • A collaborative approach including a dedicated project manager to ensure the project meets your needs
  • Development of genomically validated isogenic cell line pairs (all cell lines are provided with a non-edited control)
  • Initial characterization of parental cell line prior to gene-editing (if required)
Case Studies

Case study I: CRISPR-Mediated Homozygous Knockout

Purpose

Disruption of the gene X in the human melanoma A375 cell line (copy number = 3).

Workflow
  • Transfection with plasmids expressing Cas9 nuclease and a validated gRNA
  • Derivation of single cell clones
  • Screening of the clones
  • Genotype analysis by Sanger sequencing
Targeting Strategy

Targeting

  • Sequencing of individual alleles of 7 clones (via molecular cloning) demonstrated that 3 clones had out of frame deletions on all 3 alleles
  • Sequencing of transcripts from individual alleles indicated out of frame deletions were also present at the RNA level for two of the three clones

Clone 1

Case Study II: Heterozygous Knockin in Leukemia Cells

Purpose

To create a heterozygous point mutation K700E (AAA -> GAA) in SF3B1 in chronic myelogenous leukemia (CML) K562 cells using rAAV.

Workflow

  • Design of rAAV targeting vectors
  • rAAV infection
  • Plating of infected clones
  • PCR and Sanger sequencing to identify KI pools
  • Derivation of single cell clones
  • Screening of the clones by PCR
  • Genotype analysis by Sanger sequencing

Vector Design

Targeting

<img alt="" src="data:image/png;base64,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

Project Plan

Phase I:

Cell Line Characterization

  • Revival, quality control and establishment of the cell line in routine culture
  • Single cell dilution test
  • Transfection/infection tests
  • Gene copy number analysis

Phase II:

Gene-editing Reagent Design

  • Designing, manufacturing and testing CRISPR gRNAs
  • Designing and manufacturing a suitable donor (e.g. AAV, oligonucleotide, plasmid) if needed
  • Designing a suitable screening strategy to identify correctly targeted cells

Phase III:

Cell Line Engineering

  • Introduction of the targeting event
  • Enrichment of the targeted cell population
  • Assessment of targeting in the pools of cells
  • Plating the cells to 96-well plates and growing them for screening
  • Screening followed by in depth genetic assessment to select correctly targeted populations
  • If required, single cell dilution of targeted populations to get targeted clones
  • Expansion of the selected clones and banking

Phase IV:

Cell Line Validation

  • Final sequence validation
  • Recovery viability testing
  • Sterility testing (bacteria and mycoplasma)

Phase V:

Project Delivery

  • Data pack summarizing the process and analysis of generated cell lines; up to 2 clones per cell line

Contact Us

iPSC

Create translational in vitro disease relevant models

Learn More

CHO

Customized gene editing of the CHO genome to optimize your bioproduction platform.

Learn More

Immuno-oncology

See our extensive panel of pre-characterized immune cell lines

Learn More

Back to top