Kinase inhibition through gene edited cell lines

What Are Kinases?

Definition: Kinases are enzymes that mediate the transfer of phosphate groups to substrates. Phosphorylation of a substrate can alter its ability to interact with other molecules and its activity. For this reason kinases are important in numerous processes including; signalling, metabolism and transport among many others.

Overview of the kinase family

Kinases are a large family of enzymes with over 500 encoded in the human genome and all share a highly conserved kinase domain. Dependent on the substrate they act upon they can be broadly classified into 3 categories: protein kinases, carbohydrate kinases and lipid kinases. Due to the diverse roles that kinases play in the cell they are implicated in a number of diseases including cancer:

  • Protein kinases

    can phosphorylate substrate proteins on serine, threonine, tyrosines or histidine residues. Mitogen-activated protein kinases such as RAF kinases involved in the MAPK signalling pathways and cyclin dependent kinases (CDKs) involved in cell cycle regulation are prime examples of pathways implicated in cancers.
  • Lipid kinases

    alter the localisation and reactivity of lipids through phosphorylation. A well-known example of a lipid kinase is phosphoinositide 3-kinase which is implicated in a number of cancers, mainly through alterations to its catalytic subunit - PIK3CA.
  • Carbohydrate kinases

    such as hexokinase play important roles in the majority of metabolic pathways.
Available knockout
cell lines

Popular Knockout Cell Lines

AAK1CLK1HIPK2MAPK9PKN3SRPK2
AATKCLK2HIPK3MAPKAPK2PLK2STK10
ABL2CLK3HUNKMAPKAPK3PNCKSTK16
ACVR1CLK4ICKMAPKAPK5PRKAA1STK17A
ACVR1BCSKIGF1RMARK1PRKAA2STK17B
ACVR2BCSNK1DIKBKBMARK2PRKACASTK19
ADCK2CSNK1EINSRMARK3PRKACBSTK25
ADCK3CSNK1G1IRAK1MARK4PRKCASTK3
ADCK4CSNK1G2IRAK2MAST1PRKCDSTK32B
ADCK5CSNK1G3IRAK3MAST2PRKCESTK32C
ADRBK1CSNK2A1IRAK4MAST3PRKCGSTK33
ADRBK2CSNK2A2ITKMAST4PRKCISTK38
AKT1DAPK1JAK1MATKPRKCQSTK38L
AKT2DAPK2JAK2MELKPRKCZSTK39
ALKDAPK3KIAA1804METPRKD1STK4
ALPK1DCLK2KSR1MINK1PRKD2STK40
ALPK2DDR1KSR2MKNK1PRKD3STRADA
ARAFDDR2LATS1MKNK2PRKG2STRADB
AXIN1DMPKLATS2MLKLPRKXSTYK1
AXLDSTYKLIMK2MOKPSKH1SYK
BCKDKDYRK1BLMTK2MST4PTK2BTAF1L
BCRDYRK2LMTK3MYLKPTK7TAOK1
BMP2KDYRK3LRRK1MYLK3PXKTAOK2
BMPR1ADYRK4LRRK2MYT1RAF1TAOK3
BMPR1BEEF2KLTKNEK1RETTBCK
BMPR2EIF2AK1LYNNEK10RIOK3TBK1
BRD2EIF2AK2MAKNEK2RIPK1TEC
BRD3EIF2AK3MAP2K1NEK3RIPK2TESK1
BRDTEIF2AK4MAP2K2NEK4RNASELTESK2
BRSK1EPHA2MAP2K3NEK7ROCK1TGFBR1
BRSK2EPHA3MAP2K4NEK8ROCK2TGFBR2
CAMK1DEPHA4MAP2K5NEK9ROR1TLK1
CAMK2DEPHA6MAP2K6NLKROR2TNIK
CAMK2GEPHB1MAP2K7NRBP2RPS6KA1TNK1
CAMKK1EPHB2MAP3K1NTRK1RPS6KA2TRIB1
CAMKK2EPHB3MAP3K10NUAK1RPS6KA3TRIB2
CAMKVEPHB4MAP3K11OBSCNRPS6KA4TRIB3
CASKEPHB6MAP3K12OSR1RPS6KA6TRIO
CDC42BPAERBB2MAP3K14PAK1RPS6KB1TSSK2
CDC42BPBERBB3MAP3K2PAK2RPS6KB2TSSK3
CDC42BPGERN1MAP3K3PAK4RPS6KC1TSSK4
CDK10FASTKMAP3K4PAK6RPS6KL1TTBK2
CDK13FERMAP3K5PASKRYKTWF2
CDK14FESMAP3K6PBKSBK1TYK2
CDK16FGFR2MAP3K7PDGFRASCYL1TYRO3
CDK17FGFR3MAP3K8PDIK1LSCYL2UHMK1
CDK18FGFR4MAP3K9PDK1SCYL3ULK1
CDK19FLT1MAP4K3PDK2SGK223ULK3
CDK2FLT4MAP4K4PDK3SGK3ULK4
CDK3FYNMAP4K5PEAK1SGK494VRK1
CDK4GAKMAPK1PHKG1SIK1VRK2
CDK5GRK4MAPK11PHKG2SIK2VRK3
CDK8GRK5MAPK12PIM1SIK3WNK2
CDKL1GRK6MAPK15PIM2SLKWNK3
CDKL3GSK3AMAPK3PIM3SNRKZAK
CDKL5GSK3BMAPK4PINK1SPEG 
CHEK2HCKMAPK7PKDCCSRC 
CHUKHIPK1MAPK8PKN1SRPK1 
AGC
Subfamily
CAMK
Subfamily
CK1
Subfamily

CK1

Examples of available Knockout Cell Lines

CK1DCK1G2SGK493VRK1
CK1ECK1G3SLOBVRK2
CK1G1HaspinTTBK2VRK3
CMGC
Subfamily
STE
Subfamily
TK
Subfamily
TKL
Subfamily

Our cell line range is constantly growing. For our most current list of ready-made cell lines and details of custom made engineering projects you can browse our entire offering via the link below.

Browse Our Cell Line Options


For offline access, many of our popular cell lines that are usually available for immediate delivery are listed in our downloadable our catalog - your gene edited cell line may already have been created!

Horizon Cell Lines Catalog

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