DNA Damage Pathways

Overview of DNA damage repair mechanisms

DNA is constantly exposed to a variety of damaging factors such as genotoxic agents, environmental factors (e.g. UV light, irradiation) or normal metabolic activities (e.g. nucleotide misincorporation during DNA replication, reactive oxygen species) that causes a range of lesions. DNA repair pathways are constantly active in the cell to identify and correct all DNA damage that may occur. The wide diversity of DNA lesions requires multiple, largely distinct DNA repair processes.

The DNA repair machinery is vital for the maintenance of genome integrity and for the proper function and survival of all organisms.  A cell that has accumulated a large number of lesions can either undergo apoptosis, senescence, or in the case of cancers; unregulated cell division.  A failure to repair DNA lesions can result in mutagenesis, large scale genome aberrations and cellular cytotoxicity. 

This collection has been generated in close collaboration with Joanna I. Loizou (CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria).

Examples of popular knockout cell lines
ALKBH2 FANCI PARP1 PRKDC
APTX LIG4 PARP2 RAD52
BLM MGMT PNKP RECQL4
CHEK2 MLH1 POLB RIF1
DCLRE1C MPLKIP POLE RNF8
ERCC6 MSH2 POLH SETMAR
ERCC8 MSH3 POLI TDP1
FANCA MSH5 POLK TP53BP1
FANCB MSH6 POLL TREX1
FANCC NEIL1 POLM WRN
FANCE NHEJ1 POLN XPA
FANCG OGG1 POLQ XRCC4

Our cell line range is constantly growing. For our most current list of ready-made cell lines and details of custom made engineering projects you can browse our entire offering via the link below.

Browse Our Cell Line Options


For offline access, many of our popular cell lines that are usually available for immediate delivery are listed in our downloadable our catalog - your gene edited cell line may already have been created!

Horizon Cell Lines Catalog

 

Case study

The response of LIG4 (LIGIV) and PRKDC (DNA-PKc) (-/-) cell lines to treatment with Etoposide was studied. These proteins play a role in Non-Homologous End Joining (NHEJ) and they are both associated with disease; LIG4 is associated with T cell leukemia whilst loss of PRKDC has been linked to gastric tumors. 

LIG4 and PRKDC (-/-) cells show selective sensitivity to Etoposide over HCT116 Parental cells in an 8 day colony forming assay and a 96 hour proliferation assay. 

Download the complete application note

 Cell lines used:

Cell LineGenotypeCat. No.

HCT116

LIG4 (-/-)

HD R02-063

HCT116

PRKDC (-/-)

HD R02-049

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