Cell Lines For Studying Deubiquitinating Enzymes (DUBs)

Ubiquitin is a small protein that is attached to other proteins by the means of ubiquitin ligases. Dependent on the number of ubiquitin molecules that are added in a row and the nature of their linkage, proteins are either marked for degradation or altered in their activity, subcellular localization or protein-protein interaction. Ubiquitination is reversible and the removal of ubiquitin is catalysed by deubiquitinating enzymes.  The human genome contains 95 putative DUB genes, which can be broadly classified as cysteine proteases and metalloproteases.  Not all 95 DUBs are predicted to be functional enzymes.

The ubiquitin pathway has been implicated in a number of genetic disorders and diseases including cancer and neurological diseases which result from the build-up of protein aggregates such as; Alzheimer’s disease, Parkinson’s disease and Huntingdon’s disease.

This collection has been generated in close collaboration with Prof. Steve Jackson (Gurdon Institute, Cambridge, United Kingdom).

Experimental validation 
Deubiquitination of FANCD2 by USP1 is impacted in a USP1 knockout cell lines. Cells were washed with PBS, lysed in Frackelton buffer (10 mM Tris/HCl pH 7.5, 50 mM NaCl, 30 mM sodium pyrophosphate, 1 % Triton X-100, 50 mM NaF and protease inhibitors) and analyzed by SDS-PAGE and Western blotting using anti-FANCD2 (sc-20022 from Santa Cruz). HAP1 wild-type cells were compared to cells bearing a frameshift mutation in USP1.
Examples of popular knockout cell lines
ATXN3 OTUD5 USP18 USP4
BRCC3 OTUD6B USP19 USP40
COPS5 OTUD7B USP2 USP42
COPS6 PAN2 USP20 USP45
COPS7A STAMBP USP21 USP46
COPS7B STAMBPL1 USP22 USP47
CYLD TDG USP24 USP48
EIF3H UCHL1 USP25 USP49
JOSD1 UCHL3 USP27X USP53
JOSD2 UNG USP28 USP54
MPND USP1 USP3 USP6
MYSM1 USP10 USP30 USP7
OTUB1 USP11 USP31 USP8
OTUB2 USP13 USP32 USP9X
OTUD1 USP14 USP33 VCPIP1
OTUD3 USP15 USP34 ZRANB1
OTUD4 USP16 USP38  

Our cell line range is constantly growing. For our most current list of ready-made cell lines and details of custom made engineering projects you can browse our entire offering via the link below.

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For offline access, many of our popular cell lines that are usually available for immediate delivery are listed in our downloadable our catalog - your gene edited cell line may already have been created!

Horizon Cell Lines Catalog

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