Cell Lines For Studying Deubiquitinating Enzymes (DUBs)

Ubiquitin is a small protein that is attached to other proteins by the means of ubiquitin ligases. Dependent on the number of ubiquitin molecules that are added in a row and the nature of their linkage, proteins are either marked for degradation or altered in their activity, subcellular localization or protein-protein interaction. Ubiquitination is reversible and the removal of ubiquitin is catalysed by deubiquitinating enzymes.  The human genome contains 95 putative DUB genes, which can be broadly classified as cysteine proteases and metalloproteases.  Not all 95 DUBs are predicted to be functional enzymes.

The ubiquitin pathway has been implicated in a number of genetic disorders and diseases including cancer and neurological diseases which result from the build-up of protein aggregates such as; Alzheimer’s disease, Parkinson’s disease and Huntingdon’s disease.

This collection has been generated in close collaboration with Prof. Steve Jackson (Gurdon Institute, Cambridge, United Kingdom).

Experimental validation 
Deubiquitination of FANCD2 by USP1 is impacted in a USP1 knockout cell lines. Cells were washed with PBS, lysed in Frackelton buffer (10 mM Tris/HCl pH 7.5, 50 mM NaCl, 30 mM sodium pyrophosphate, 1 % Triton X-100, 50 mM NaF and protease inhibitors) and analyzed by SDS-PAGE and Western blotting using anti-FANCD2 (sc-20022 from Santa Cruz). HAP1 wild-type cells were compared to cells bearing a frameshift mutation in USP1.
Examples of popular knockout cell lines
ATXN3OTUD5USP18USP4
BRCC3OTUD6BUSP19USP40
COPS5OTUD7BUSP2USP42
COPS6PAN2USP20USP45
COPS7ASTAMBPUSP21USP46
COPS7BSTAMBPL1USP22USP47
CYLDTDGUSP24USP48
EIF3HUCHL1USP25USP49
JOSD1UCHL3USP27XUSP53
JOSD2UNGUSP28USP54
MPNDUSP1USP3USP6
MYSM1USP10USP30USP7
OTUB1USP11USP31USP8
OTUB2USP13USP32USP9X
OTUD1USP14USP33VCPIP1
OTUD3USP15USP34ZRANB1
OTUD4USP16USP38 

Our cell line range is constantly growing. For our most current list of ready-made cell lines and details of custom made engineering projects you can browse our entire offering via the link below.

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For offline access, many of our popular cell lines that are usually available for immediate delivery are listed in our downloadable our catalog - your gene edited cell line may already have been created!

Horizon Cell Lines Catalog

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