Horizon can tailor a custom cell line to your exact specifications
If you can't find your cell line of interest in our catalog, we can engineer a custom cell line for you. We use our cell line gene-editing platform to create a custom cell line to meet your exact specifications, whether that be a knockout, knockin, deletion, translocation or reporter cell line. Your custom cell line will be always delivered to you with its non-edited control.
Use our custom cell lines to:
Horizon’s team has 10 years of experience in genome-editing using CRISPR, rAAV and Zinc Finger Nuclease (ZFNs) technologies which allows us to complete complex gene-editing projects in agreed timelines. If we haven’t engineered the cell line of your interest yet, our cell line characterization and optimization service will be included to maximize success of your gene editing project.
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From 10 weeks |
From 13 weeks |
From 20 weeks |
*Knockouts by NHEJ-mediated frame-shift mutations. Defined deletions and gene disruption via targeted insertion are subject to knockin pricing. Some cell lines may be polyploid at some loci. Additional alleles may be modifed for an additional fee. **Possessed by Horizon or in ATCC for the commercial use. ***Characterization of more than one cell line is available for an additional fee. 1Cell line must tolerate single cell cloning and display adequate transfection efficiency. ^KI of additional alleles is available for an additional fee. #A plasmid donor carrying a selection cassette will be utilized.
Learn more about our gene-editing capabilities in induced pluripotent stem cells (iPSCs) and immune cells. |
Case studies
Case study I: CRISPR-Mediated Homozygous Knockout | |
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Purpose: Disruption of the gene X in the human melanoma A375 cell line (copy number = 3) | |
Workflow
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Targeting Strategy
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Targeting
Clone 1 |
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Case Study II: Heterozygous Knockin in Leukemia Cells | |
Purpose: To create a heterozygous point mutation K700E (AAA -> GAA) in SF3B1 in chronic myelogenous leukemia (CML) K562 cells using rAAV | |
Workflow
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Vector Design |
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Targeting Targeted allele-specific sequencing shows that both clones contain the K700E mutation. Non-allele specific sequencing shows a ratio of 50:50 of the mutant to wild-type peaks, as would be expected for a 2N locus. |
What else can Horizon do to support your project? | |||
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Cell Line Characterization
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Functional Validation
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Other Assay Services
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In Vivo Studies
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Request more information about our assays
Related materials
Watch our webinar on gene editing | Click here |
Read our blog post on improving gene-editing success with optimizing cell line screening procedure | Click here |
Learn how panels of engineered cell lines can be used for predicting patient drug responses | Click here |