EGFR Gene-Specific Multiplex Reference Standard 5% FFPE


The Gene-Specific FFPE Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations. These standards are available specifically in either an EGFR Multiplex (1 and 5% allelic frequency range) or a KRAS Multiplex (5% allelic frequency range). With this product you are able to: • Evaluate of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics • Analyze the sensitivity of your workflow • Optimize and validate new cancer panels and routinely monitor the performance of your assay

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Technical Information
Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Gene-Specific Multiplex Reference Standard portfolio covers multiple endogenous SNPs, insertions and deletions at defined allelic frequency ranges. The Gene-Specific Reference Standards cover EGFR or KRAS-specific mutations. These standards may be diluted with a matched wild type while the background mutations remain consistent. The Gene Specific Multiplex Standards are available in genomic DNA and FFPE format, which allows you to validate both the pre-analytical and analytical portion of your workflow. The FFPE format allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples.

By multiplexing multiple gene-specific mutations in a single standard, the Gene-Specific Multiplex allows researchers to maximize the information obtained from their assay, and confirm that they are able to detect a range of potential mutants in a single gene.

Technical Data

Genes Covered
Fixation Method
4% Formalin
Section Size
15µm or 20µm
Cell Density
3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield
≥ 400 ng using Promega Maxwell LEV Plus Extraction kit

Product Information

Verified Mutations
ChromosomeGeneVariantExpected Allelic Frequency, %
7p12EGFRΔE746 - A7505.00%

Presence confirmed in parental cell line

ChromosomeGeneVariantExpected Allelic Frequency, %
Unit Size
1 FFPE Section
Extractable DNA
≥ 400 ng per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit
Intended Use
For routine performance monitoring (Research Use Only)

General Information

Allelic Frequency
48 months from the date of manufacture
Cell Line Background


Quality Control

Sanger sequencing of locus specific PCR
Agarose gel electrophoresis
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

PDF Download
Batch ID: 35654
PDF Download

Documents & Related Literature

Safety Data Sheet
PDF Download
Order & Delivery Information

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If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:


Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.


If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.

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