Biomanufacturing Resources

CHO Guevara BioreactorProtocols

Our cell culture protocol download will assist in the use of our GS null selection system for your stable cell line development. It includes instructions on:

  • Thawing and subculturing
  • Transfection (lipid based and electroporation)
  • Selection using the GS selection system
  • Small scale and bioreactor culturing

Cell Culture Protocol

Example clonal expression dataData

Our latest product data sheet is available for download, it contains expression data from a variety of evaluations that used GS selection as part of the CHO SOURCE GS null CHO K1 expression system to generate recombinant proteins.

Alternatively, see our technical information:

Product Sheet Technical Data

Molecular validation of knockoutSafety

Visit the relevant section of the website relating to information on how the GS Null CHO K1 cell line was generated. This section includes:

  • Origins and acquisition of the CHO K1 cell line
  • Engineering of the adherent cells
  • Genomic and functional characterisation of Glutamine Synthetase knockout
  • Adaptation to suspension growth in animal component free, chemically defined media
  • cGMP banking

Cell Line Derivation

 

Download a copy of the document we sent to the MHRA prior to a meeting requesting Scientific Advice. This document includes:

  • Background information on the engineering and derivation of the cell line
  • The questions we put to the MHRA regarding their opinions on our culturing, engineering and traceability of the cell line
  • The responses the MHRA gave to these questions

MHRA Scientific Advice

White papers

Download our white paper on the role genome engineering has to play in the future of bioprocessing and biomanufacturing. This whitepaper discusses:

  • How to improve the expression capacity of biomanufacturing during cell line development of CHO cells
  • The different genome engineering methods available to modify CHO cells for improved recombinant protein expression
  • How improved manufacturing can drive personalized medicine
  • The importance of metabolic selection systems such as DHFR or Glutamine Synthetase

Maximising the potential of biomanufacturing cell lines

Click here to read our tutorial that was published in GEN on how genome engineering has been used to remove host cell proteins that were co-purifying with a recombinant protein product and could not easily be removed through downstream processing approaches.

Engineering Biotherapeutic Quality

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