Tru-Q 2 (5% Tier) Reference Standard

Format
Genomic DNA

Description
The Tru-Q DNA Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations. With this product you are able to: • Analyze the sensitivity and specificity of your assay • Gain certainty of the limit of detection and limit of quantification • Optimize and validate new cancer panels and routinely monitor the performance of your assay
HD729
$255.00

Availability: In stock

Dispatched within 24 hours

Bulk order discount
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Technical Information
Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Tru-Q DNA Reference Standard portfolio covers multiple endogenous SNPs, insertions and deletions. Tru-Q NGS DNA Reference Standard 1 (5% Tier) covers 10 mutations at 5% allelic frequency in genomic DNA format. These may be diluted to even lower allelic frequencies using our Tru-Q 0 Wild Type standard. Furthermore, because the Tru-Q series has 4 different standards at the 5% alleleic frequency range, you may use rotate these as “blinded” samples for proficiency testing in your laboratory.

These standards are available only in gDNA format.

Technical Data

Genes Covered
ALK, BRAF, EGFR, FGFR2, GNAQ, IDH2, KRAS, NRAS, PIK3CA
Cosmics
1,500+
Allelic Frequencies
5% for verified variants
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

Product Information

Verified Mutations

The Tru-Q Reference Standard has been manufactured using ten engineered cell lines and mixed to generate a 5% Allelic Frequency multiplex sample:

ChromosomeGeneVariantExpected Allelic Frequency, %
2p23ALKF1174L5.0%
7q34BRAFV600E8.0%
7q34BRAFV600G4.0%
7p12EGFRG719S16.7%
7p12EGFRL858R4.2%
10q26FGFR2S252W4.0%
9q21GNAQQ209L5.0%
15q26.1IDH2R140Q5.0%
12p12.1KRASG12V3.8%
12p12.1KRASG13D25.0%
12p12.1KRASQ61L5.0%
1p13.2NRASQ61L5.0%
3q26.3PIK3CAE545K5.0%
3q26.3PIK3CAH1047R30.0%
Unit Size
1µg
Concentration
50ng/µl

General Information

Storage
4˚C
Expiry
36 months from the date of manufacture
Cell Line Background

HCT116/RKO/SW48

Quality Control

Allelic Frequency
Droplet Digital PCR™
Genotype
Sanger sequencing of locus specific PCR
Quality
Agarose gel electrophoresis
Quantification
Spectrophotometry (A260)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: gDNA-multiplex
PDF Download
Batch ID: 34567
PDF Download

Documents & Related Literature

Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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