What other validations do you recommend to be performed?

The ultimate proof that a cell line does not express the gene of interest is protein expression analysis by Western blot or similar. Unfortunately, due to the costs and time involved in optimising any new antibody, Horizon cannot test protein expression in all its cell lines. As such we recommend that protein expression is assessed in cell lines prior to proceeding with downstream experiments.

Please contact us if a HAP1 knockout cell line retains gene expression as detected by Western blotting.

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