Given the time intensive nature of gene engineering, the relatively straightforward and quick (<1 week) process of gRNA validation can save weeks of cell culture and hours of bench time. Further to this, a clear idea of gRNA activity will provide insights into how many clones need to be screened to identify a positive.
There are now a variety of methodologies available to scientists looking to compare and validate guide RNA activity. Six of the most commonly used approaches are:
You'll find our complete guide to these approaches as well as tips for the Surveyor assay in our article here: