Functional Observational Battery

Basic phenotyping conducted by experts so you can interpret your data with confidence

Horizon Discovery offers the functional observational battery (FOB) as part of our Phenotyping Platform. This important phenotypic assay is performed by our partner, InterVivo Solutions, a leader in rodent behavioral assays. Evaluating animals for gross physiological and neurological deficits, the FOB is based upon the gold-standard Irwin test, and serves as an initial phenotypic characterization of your transgenic line. It is important to conduct a proper FOB to ensure that an undetected physiological or neurological defect does not jeopardize a laboratory animal’s ability to perform key tasks or confound data interpretation.

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Figure 1. Sample Pathfinder FOB performed on Pink1 knockout rats and wild type control rats at 4, 5, and 6 months of age. Red denotes increases and blue decreases/deficits.

Modified Irwin Testing Battery

Modified Irwin Testing, as conducted by InterVivo Solutions, includes a multitude of parameters based upon the original definition by Samuel Irwin of the University of Oregon (Irwin 1968).

Home Cage Observations

  • Body posture
  • Respiration
  • Coat appearances
  • Vocalization
  • Convulsion
  • Arousal
  • Ptosis/Eyelid Position

Open Field Observations

  • Mobility/Gait
  • Chewing
  • Rearing
  • Twitching/Tremors
  • Alertness
  • Sterotypic behavior
  • Defecation
  • Various responses and reflexes

Handheld Observations

  • Ease of handling
  • Abdominal tone
  • Eye blink response
  • Lacrimation
  • Salivation
  • Eye and skin color
  • Body temperature

Pathfinder Functional Observational Battery (FOB) uses:

Phenotypic Assessment

The Functional Observational Battery is an initial phenotypic characterization of a transgenic animal, and can be performed on:

  • SAGEspeed Custom Models
    Jump right into your experiments and hit the ground running with your new model. There is no need to set up and learn new characterization assays in your lab or spend the time performing this initial characterization.
  • SAGE Catalog Research Models
    Hit the ground running with a deeper understanding of our catalog models.
  • Transgenic Rat or Mouse Models
    Simply send us your model and we’ll take care of the rest.

Neurotoxicity Screening

The FOB assesses the potential neurotoxicity of compounds and small molecule therapeutics. Neurotoxicity screening with the Pathfinder FOB can be performed on:

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