The discovery of CRISPR genome editing has provided a novel and highly efficient way to probe gene function through the generation of gene knockouts.
Horizon’s CRISPR KO screening service is using a high performance vector system. The all-in one vector system offers significantly improved performance in a rapid workflow (Cross et al., 2016). Our optimized screening platform, sophisticated bioinformatics pipeline and multiple ready-made and custom libraries allow us to design the most appropriate workflow for your screening project to provide you with data of exceptional quality.
Horizon can provide complete workflows for CRISPR knockout screens, from cell line selection and optimisation through to bioinformatics analysis of the screen results.
Turnaround time: 12-20 weeks
Deliverables: Data in raw and analysed form and a final report containing experimental details and hit nominations
Select one of our off-the shelf sgRNA libraries or have us design a custom library!
To learn more about our CRISPR screening platform, watch our recorded webinar:
Purpose: A proof of concept study to identify resistance factors against a BRAF kinase inhibitor Vemurafenib (PLX-4032) in A375 melanoma cells that carry a BRAF V600E gain-of-function mutation.
Figure 1. Ranking of the hits of the screen by MAGeCK algorithm
Download the complete application note: