TruSeq Amplicon

What is Amplicon Sequencing?

Definition: An amplicon is one or more copies of a genetic fragment or target sequence as a product of natural or artificial amplification. Amplicon sequencing is a targeted method of analysis of these amplicons often carried out using Polymerase chain reaction (PCR) or Next-generation sequencing.

Below is a summary of genes covered by the TruSeq Amplicon panel, plus our recommendations for which Horizon DIscovery reference standard is most suitable for each panel (based on gene coverage).

Genes Covered By TruSeq Amplicon Panel

ABL1
ALK
APC
BRAF
CDH1
CTNNB1
EGFR
FBXW7
FGFR1
FGFR2
FLT3
GNA11
GNAQ
IDH1
JAK2
KIT
KRAS
MET
MLH1
NOTCH1
NRAS
PDGFRA
PIK3CA

Recommended Reference Standards:

Quantitative Multiplex (>16 genes covered)

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Tru-Q (>21 genes covered)

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