CD274 (PD-L1) Expression IHC Reference Standard [DISCONTINUED]

B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Format Type
Biomarker Specific

FFPE section on slide

The CD274 (PD-L1) Reference Standard is a highly-characterized, biologically-relevant quality control material with negative (-), low positive (+), intermediate positive (++) and strong positive (+++) controlled protein expressing cell lines which can be utilised to test the analytical (technical) performance of your assay.

Availability: Discontinued

Bulk order discount
Technical Information
The availability of precisely defined reference materials offers an industry standard for development and quality control of IHC assays, improving the accuracy and reproducibility. IHC HDx Reference Standards provide laboratories with a consistent source of positive and negative reference material on one slide.

• Precisely defined negative (-), low positive (+), intermediate positive (++) and strong positive (+++) protein expressing cell lines on the same slide (Onslide control)
• Cell lines verified by IHC assay and Quantitative Digital Pathology (QDP).

• Test the analytical (technical) performance of your assay with external controls of precisely defined human cell lines that express protein at a range of known levels
• Establish the practical lower limit of detection of your IHC methodology with validated low protein expressing cells
• Develop a new IHC protocol with a reproducible assay that is validated through reproducible cell lines
• Guide the evaluation of antibodies based on their sensitivity and specificity
• Gain additional confidence when optimizing and monitoring the performance of your assay through application of a consistent reference standard

Technical Data

The following tests are run as standard to ensure the quality of our IHC Reference Standards:
• Protein expression confirmed using biomarker specific IHC assay.
• Negative (-), low positive (+), intermediate positive (++) and strong positive (+++) cell line cores assessed using Quantitative Digital Pathology
Mutation Type
Fixation Method
10% Formalin
Section Thickness
Core Diameter
Cell Density
1-1.5 x 104 cells/core dependent on cell size
Microscope Slide
Superfrost Plus

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
5 slides per pack

General Information

28 months


Core 1
Core 2
Core 3
Core 4
Core Legends

Typical Staining Pattern of PD-L1 Expression
Core 1: No membrane staining pattern is observed in the negative core (-)
Core 2: Low positive membrane staining pattern is observed in the low positive core (+)
Core 3: Intermediate positive membrane staining pattern is observed in the intermediate positive core (++)
Core 4: Strong positive membrane staining pattern is observed in the strong positive core (+++)
(Cell Signaling, E1L3N, #13684 on the automated Leica BOND Max stainer)

Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 18228
PDF Download
Batch ID: 21875
PDF Download
Batch ID: 24012
PDF Download
Batch ID: 24013
PDF Download

Documents & Related Literature

Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:


Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.


If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada



FEDEX International Priority

EU Countries
(Excluding Italy)



FEDEX International Priority




Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 


$25.00 - $80.00


$45.00 - $120.00


FEDEX International Priority


We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering


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