Multiplex I cfDNA Reference Standard Set in Synthetic Matrix

Format
cfDNA

Description

Developed using clinically-relevant mutations in an artificial matrix to give you full control of your cfDNA workflow, reducing the likelihood of a false negative or positive due to incorrect assay loading. Multiplex I cfDNA Reference Standard Set in synthetic matrix contains 8 onco-relevant mutations to measure your extraction efficiency. Put the control of your workflow back in your hands.

Catalog ID
Qty
Unit Price
HD817
$575.00
Bulk order discount

Availability: In stock

Dispatched within 24 hours

Total:
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Technical Information

The Multiplex I cfDNA Reference Standard Set in artificial matrix covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.

For information on specific gene copies detected in each product batch, download the product insert from the Protocols and Documents tab.


Technical Data

Genes Covered
EGFR, KRAS, NRAS, PI3KCA
Cosmics
1,500+
Allelic Frequencies
5%, 1%, 0.1% and 0% (100% wild type)
Buffer
Synthetic matrix
Expected DNA Yield
≥120 ng DNA using the QIAamp® Circulating Nucleic Acid Kit

Product Information

Fragment Size
170bp
Unit Size
400ng per 1ml (4 vials total)
Verified Mutations
100% Multiplex I Wild Type cfDNA Reference Standard (Part No.: HD815)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.00%
7p12EGFRΔE746 - A7500.00%
7p12EGFRT790M0.00%
7p12EGFRV769 - D770insASV0.00%
12p12.1KRASG12D0.00%
1p13.2NRASQ61K0.00%
1p13.2NRASA59T0.00%
3q26.3PIK3CAE545K0.00%
5% Multiplex I cfDNA Reference Standard  (Part No.: HD812)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R5.0%
7p12EGFRΔE746 - A7505.0%
7p12EGFRT790M5.0%
7p12EGFRV769 - D770insASV5.0%
12p12.1KRASG12D6.3%
1p13.2NRASQ61K6.3%
1p13.2NRASA59T6.3%
3q26.3PIK3CAE545K6.3%
1% Multiplex I cfDNA Reference Standard   (Part No.: HD813)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R1.0%
7p12EGFRΔE746 - A7501.0%
7p12EGFRT790M1.0%
7p12EGFRV769 - D770insASV1.0%
12p12.1KRASG12D1.3%
1p13.2NRASQ61K1.3%
1p13.2NRASA59T1.3%
3q26.3PIK3CAE545K1.3%
0.1%  Multiplex I cfDNA Reference Standard (Part No.: HD814)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.10%
7p12EGFRΔE746 - A7500.10%
7p12EGFRT790M0.10%
7p12EGFRV769 - D770insASV0.10%
12p12.1KRASG12D0.13%
1p13.2NRASQ61K0.13%
1p13.2NRASA59T0.13%
3q26.3PIK3CAE545K0.13%
Presence confirmed in parental cell line

EGFR G719S (20% allelic frequency); download additional information on variants present in the parental cell lines:

Exome Sequencing Data

General Information

Storage
4°C
Expiry
Stable for 24 months from date of manufacture (as supplied)
Cell Line Background

RKO/SW48

Quality Control

Fragmentation Size
D1000 DNA ScreenTape assay
Allelic Frequency
Droplet Digital™ PCR
Quantification
Qubit dsDNA BR Assay (post-fragmentation)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: cfDNA-matrix
PDF Download

Documents & Related Literature

Product Insert
PDF Download
Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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