5 Fusion Multiplex RNA Negative Control



The 5-Fusion Multiplex (Negative Control) FFPE RNA Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of targeted NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions. Each FFPE section is composed of a formalin-fixed, paraffin-embedded (FFPE) cell line verified not to contain EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusions.

With this product you are able to:
• Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
• Negative control for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 fusion detection panels


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Technical Information

Targeted NGS, endpoint RT-PCR and RT-qPCR platforms give researchers much greater power when profiling tumor samples. However, they present challenges surrounding sample variability, RNA extraction procedures, RNA quantification, platform bias, and potential failure to detect biomarkers.

Technical Data

Genes Covered
Negative for EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3
Cosmic ID
COSF463 (EML4-ALK); COSF1272 (CCDC6-RET); COSF1197 (SLC34A2-ROS1); COSF1330 (TPM3-NTRK1); COSF572 (ETV6-NTRK3)
Fixation Method
10% Formalin
Section Size

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
1 FFPE Section

General Information

12 months from the date of manufacture

Quality Control

Extractable RNA / Expected Yield
≥ 100 ng RNA per section using the Maxwell 16 LEV RNA FFPE Purification Kit from Promega
Biomarker-specific mRNA amplifiability
Absence of EML4-ALK, CCDC6-RET, SLC34A2-ROS1, TPM3-NTRK1 and ETV6-NTRK3 RNA fusion transcripts confirmed by endpoint RT-PCR
TATA-binding protein (TBP) mRNA amplifiability control
RNA expression confirmed by endpoint RT-PCR
Qubit RNA HS kit from ThermoFisher as per the manufacturer’s instructions
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 18053
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Batch ID: 38486
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Documents & Related Literature

Safety Data Sheet
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