Multiplex I cfDNA Reference Standard Set in Synthetic Plasma

Format
cfDNA

Description

Developed using clinically-relevant mutations in synthetic plasma to give you full control of your cfDNA workflow, reducing the likelihood of a false negative or positive due to incorrect assay loading. Multiplex I cfDNA Reference Standard Set in synthetic plasma contains 8 onco-relevant mutations to measure your extraction efficiency. Put the control of your workflow back in your hands.

HD816
$1,150.00

Availability: In stock

Dispatched within 24 hours

Bulk order discount
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Technical Information

The Multiplex I cfDNA Reference Standard Set in synthetic plasma covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.

For information on specific gene copies detected in each product batch, download the product insert from the Protocols and Documents tab.


Technical Data

Genes Covered
EGFR, KRAS, NRAS, PI3KCA
Cosmics
1,500+
Allelic Frequencies
5%, 1%, 0.1% and 0% (100% wild type)
Buffer
Synthetic plasma
Expected DNA Yield
≥240 ng DNA (from 2 ml of plasma) using the QIAamp® Circulating Nucleic Acid Kit

Product Information

Fragment Size
170bp
Unit Size
400ng per 1ml (8 vials total)
Verified Mutations
100% Multiplex I Wild Type cfDNA Reference Standard (Part No.: HD815)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.00%
7p12EGFRΔE746 - A7500.00%
7p12EGFRT790M0.00%
7p12EGFRV769 - D770insASV0.00%
12p12.1KRASG12D0.00%
1p13.2NRASQ61K0.00%
1p13.2NRASA59T0.00%
3q26.3PIK3CAE545K0.00%
5% Multiplex I cfDNA Reference Standard (Part No.: HD812)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R5.0%
7p12EGFRΔE746 - A7505.0%
7p12EGFRT790M5.0%
7p12EGFRV769 - D770insASV5.0%
12p12.1KRASG12D6.3%
1p13.2NRASQ61K6.3%
1p13.2NRASA59T6.3%
3q26.3PIK3CAE545K6.3%
1% Multiplex I cfDNA Reference Standard (Part No.: HD813)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R1.0%
7p12EGFRΔE746 - A7501.0%
7p12EGFRT790M1.0%
7p12EGFRV769 - D770insASV1.0%
12p12.1KRASG12D1.3%
1p13.2NRASQ61K1.3%
1p13.2NRASA59T1.3%
3q26.3PIK3CAE545K1.3%
0.1% Multiplex I cfDNA Reference Standard (Part No.: HD814)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.10%
7p12EGFRΔE746 - A7500.10%
7p12EGFRT790M0.10%
7p12EGFRV769 - D770insASV0.10%
12p12.1KRASG12D0.13%
1p13.2NRASQ61K0.13%
1p13.2NRASA59T0.13%
3q26.3PIK3CAE545K0.13%
Presence confirmed in parental cell line

EGFR G719S (20% allelic frequency); download additional information on variants present in the parental cell lines:

Exome Sequencing Data

General Information

Storage
4°C
Expiry
Stable for 24 months from date of manufacture (as supplied)
Cell Line Background

RKO/SW48

Quality Control

Fragmentation Size
D1000 DNA ScreenTape assay
Allelic Frequency
Droplet Digital™ PCR
Quantification
Qubit dsDNA BR Assay (post-fragmentation)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: cfDNA-plasma
PDF Download
Batch ID: 35937
PDF Download

Documents & Related Literature

Product Insert
PDF Download
Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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