BRAF V600K Reference Standard

Synonyms
B-Raf, BRAF1

Format
FFPE

Description
The BRAF V600K Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays. With this product you are able to: • Evaluate of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics • Analyze the sensitivity of your workflow • Optimize and validate new cancer panels and routinely monitor the performance of your assay
HD274

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Technical Information
Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild type standard to generate a lower allelic frequencies for limit of detection studies and validation of your pre-analytical phase of the workflow. Horizon’s Base-Seq products are all derived from human cell lines. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly-reproducible and consistent FFPE curls. With any commercially available FFPE extraction protocol, our standards yield high molecular weight DNA. This format ensures that they may applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

Technical Data

DNA Base Change
GTG→AAG
NCBI Reference Assembly SNP
rs113488022
Cosmic ID
COSM473
Fixation Method
4% Formalin
Section Size
15µm or 20µm
Cell Density
3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield
≥ 400 ng using Promega Maxwell LEV Plus Extraction kit

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
1 FFPE Section per vial
Extractable DNA
≥ 400 ng per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit

General Information

Allelic Frequency
1%
Storage
4˚C
Expiry
48 months from the date of manufacture
Cell Line Background

HCT116

Quality Control

Allelic Frequency
Droplet Digital PCR™
Genotype
Sanger sequencing of locus specific PCR
Quality
Agarose gel electrophoresis
Quantification
Quantifluor™
Genotype
BRAF (V600K/+)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: FFPE-DNA
PDF Download

Documents & Related Literature

Safety Data Sheet
PDF Download
Gene Editing Sequence File
PDF Download
Order & Delivery Information
If you want to place an order, please contact our distributor
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