ALK-RET-ROS1 Fusion FFPE RNA Reference Standard

Format
FFPE

Description

The ALK-RET-ROS1 targeted FFPE RNA Fusion Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions. Each section contains formalin-fixed, paraffin-embedded (FFPE) cell line multiplex verified to contain EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions.

With this product you are able to:
• Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
• Optimize and validate new targeted RNA panels and routinely monitor the performance of your assay
• Assess analytical variability and reproducibility between platforms, laboratories, operators and assays and hence achieve further confidence in your clinical workflow
• Highly homogeneous cell suspension that yields a consistent and reproducible quantity of RNA that enables effective end-to-end process validation

Catalog ID
Qty
Unit Price
HD784
$105.00
Bulk order discount
  • Buy 3 for $90.00 each and save 15%
  • Buy 5 for $84.00 each and save 20%

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Technical Information

Targeted NGS, endpoint RT-PCR and RT-qPCR platforms give researchers much greater power when profiling tumor samples. However, they present challenges surrounding sample variability, RNA extraction procedures, RNA quantification, platform bias, and potential failure to detect biomarkers. To meet this need, Horizon has developed a targeted EML4-ALK, CCDC6-RET and SLC34A2-ROS1 verified cell line multiplex in an FFPE format. This renewable and highly-characterised biologically-relevant reference materials contain cell lines that were either engineered or clonally derived from a fusion background. The utilisation of clonal cell populations derived from single cell dilution and controlled manufacturing environment ensure reproducible amplifiable levels of EML4-ALKv1, CCDC6-RET and SLC34A2-ROS1 allowing for a consistent external quality control.

Applications
• Assess the performance of your RNA-seq, endpoint RT-PCR or RT-qPCR aimed at detecting EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions
• Ensure confidence in your clinical workflow, from RNA extraction to analysis
• Assess analytical variability and reproducibility between platforms, laboratories, operators and assays

Breakpoint sequence data

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Technical Data

Genes Covered
EML4-ALK, CCDC6-RET and SLC34A2-ROS1
Cosmic ID
COSF463 (EML4-ALK); COSF1272 (CCDC6-RET); COSF1197 (SLC34A2-ROS1)
Fixation Method
10% Formalin
Section Size
10µm

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
1 FFPE Section

General Information

Storage
4°C
Expiry
12 months from the date of manufacture

Quality Control

Extractable RNA / Expected Yield
≥ 100 ng RNA per section using the Maxwell 16 LEV RNA FFPE Purification Kit from Promega
Biomarker-specific mRNA amplifiability
Presence of EML4-ALK, CCDC6-RET and SLC34A2-ROS1 RNA fusion transcripts confirmed by endpoint RT-PCR
TATA-binding protein (TBP) mRNA amplifiability control
RNA expression confirmed by endpoint RT-PCR
Quantification
Qubit RNA HS kit from ThermoFisher as per manufacturer’s instructions
Protocols & Documents

Please browse our collection of downloads below.

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Certificate of Analysis

Batch ID: 17991
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Batch ID: 19883
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Batch ID: 25773
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Batch ID: 25910
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Batch ID: 27433
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Batch ID: 28091
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Documents & Related Literature

Gene Editing Sequence File
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