ALK-RET-ROS1 Fusion RNA Reference Standard



The ALK-RET-ROS1 targeted FFPE RNA Fusion Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions. Each section contains formalin-fixed, paraffin-embedded (FFPE) cell line multiplex verified to contain EML4-ALK (Variant 1), CCDC6-RET and SLC34A2-ROS1 fusions.

With this product you are able to:
• Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
• Optimize and validate new targeted RNA panels and routinely monitor the performance of your assay
• Assess analytical variability and reproducibility between platforms, laboratories, operators and assays and hence achieve further confidence in your clinical workflow
• Highly homogeneous cell suspension that yields a consistent and reproducible quantity of RNA that enables effective end-to-end process validation

5% discount on online orders

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Technical Information
Targeted NGS, endpoint RT-PCR and RT-qPCR platforms give researchers much greater power when profiling tumor samples. However, they present challenges surrounding sample variability, RNA extraction procedures, RNA quantification, platform bias, and potential failure to detect biomarkers. To meet this need, Horizon has developed a targeted EML4-ALK, CCDC6-RET and SLC34A2-ROS1 verified cell line multiplex in an FFPE format. This renewable and highly-characterised biologically-relevant reference materials contain cell lines that were either engineered or clonally derived from a fusion background. The utilisation of clonal cell populations derived from single cell dilution and controlled manufacturing environment ensure reproducible amplifiable levels of EML4-ALKv1, CCDC6-RET and SLC34A2-ROS1 allowing for a consistent external quality control.

• Assess the performance of your RNA-seq, endpoint RT-PCR or RT-qPCR aimed at detecting EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions
• Ensure confidence in your clinical workflow, from RNA extraction to analysis
• Assess analytical variability and reproducibility between platforms, laboratories, operators and assays

Technical Data

Genes Covered
EML4-ALK (Variant 1), CCDC6-RET and SLC34A2-ROS1
Cosmic ID
COSF463 (EML4-ALK); COSF1272 (CCDC6-RET); COSF1197 (SLC34A2-ROS1)
Fixation Method
10% Formalin
Section Size

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
1 FFPE Section

General Information

12 months from the date of manufacture

Quality Control

Extractable RNA / Expected Yield
≥ 100 ng RNA per section using the Maxwell 16 LEV RNA FFPE Purification Kit from Promega
Biomarker-specific mRNA amplifiability
Presence of EML4-ALK (Variant 1), CCDC6-RET and SLC34A2-ROS1 RNA fusion transcripts confirmed by endpoint RT-PCR
TATA-binding protein (TBP) mRNA amplifiability control
RNA expression confirmed by endpoint RT-PCR
Qubit RNA HS kit from ThermoFisher as per manufacturer’s instructions
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 17991
PDF Download
Batch ID: 19883
PDF Download
Batch ID: 25773
PDF Download

Documents & Related Literature

Gene Editing Sequence File
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:


Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.


If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada



FEDEX International Priority

EU Countries
(Excluding Italy)



FEDEX International Priority




Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 


$25.00 - $80.00


$45.00 - $120.00


FEDEX International Priority


We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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