ALK-RET-ROS1 Fusion Negative FFPE RNA Reference Standard

Format
FFPE

Description

The ALK-RET-ROS1 Fusion Negative FFPE RNA Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of targeted NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions. Each FFPE section is composed of a formalin-fixed, paraffin-embedded (FFPE) cell line verified not to contain EML4-ALK (Variant 1), CCDC6-RET and SLC34A2-ROS1 fusions.

With this product you are able to:
• Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
• Negative control for EML4-ALK (Variant 1), CCDC6-RET and SLC34A2-ROS1 fusion detection panels

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Catalog ID
Qty
Unit Price
HD783
$100.00
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Technical Information
Biologically-relevant quality control material used to assess the performance of targeted NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions

Technical Data

Genes Covered
Negative for EML4-ALK (Variant 1), CCDC6-RET and SLC34A2-ROS1
Cosmic ID
COSF463 (EML4-ALK); COSF1272 (CCDC6-RET); COSF1197 (SLC34A2-ROS1)
Fixation Method
10% Formalin
Section Size
10µm

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
1 FFPE Section

General Information

Storage
4°C
Expiry
12 months from the date of manufacture

Quality Control

Extractable RNA / Expected Yield
≥ 100 ng RNA per section using the Maxwell 16 LEV RNA FFPE Purification Kit from Promega
Biomarker-specific mRNA amplifiability
Absence of EML4-ALK (Variant 1), CCDC6-RET and SLC34A2-ROS1 RNA fusion transcripts confirmed by endpoint RT-PCR
TATA-binding protein (TBP) mRNA amplifiability control
RNA expression confirmed by endpoint RT-PCR
Quantification
Qubit RNA HS kit from ThermoFisher as per the manufacturer’s instructions
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 18053
PDF Download
Batch ID: 20648
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Batch ID: 21798
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Batch ID: 25792
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Documents & Related Literature

Safety Data Sheet
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